Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
1997-10-21
|
| pubmed:abstractText |
The MHC class I molecules present antigenic peptides to CTL. The peptides are delivered to the secretory pathway by TAP, which is formed by the association of MHC-encoded TAP1 and TAP2 gene products. Tumor cells incubated or transfected with IL-10 had decreased but peptide-inducible expression of MHC class I, decreased sensitivity to MHC class I-restricted CTL, and increased NK sensitivity. We here demonstrate that IL-10 expression in the murine lymphoma RMA inhibits the TAP-dependent translocation of peptides to the endoplasmic reticulum, resulting in accumulation of immature MHC class I molecules in the endoplasmic reticulum and subsequently low expression of cell surface MHC class I molecules. This finding is explained by a down-regulation of expression of TAP1 and TAP2, observed in IL-10-transfected RMA cells as well as in IL-10-transfected P815 mastocytoma cells. In the J558L plasmacytoma cell line, constitutively expressing high levels of IL-10, increased TAP-dependent translocation of peptides and expression of cell surface MHC class I could be induced by IL-10 antisense expression. IL-10 is the first example to demonstrate that a cytokine can decrease the expression and function of the TAP1/2 molecular complex and, in more general terms, the first example of a cytokine with an inhibitory effect on MHC class I-mediated Ag presentation.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ATP-Binding Cassette Transporters,
http://linkedlifedata.com/resource/pubmed/chemical/H-2 Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/TAP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/TAP2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tap1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tap2 protein, mouse
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
159
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3195-202
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9317117-ATP-Binding Cassette Transporters,
pubmed-meshheading:9317117-Animals,
pubmed-meshheading:9317117-Antigen Presentation,
pubmed-meshheading:9317117-Biological Transport,
pubmed-meshheading:9317117-Down-Regulation,
pubmed-meshheading:9317117-Endoplasmic Reticulum,
pubmed-meshheading:9317117-H-2 Antigens,
pubmed-meshheading:9317117-Immunity, Innate,
pubmed-meshheading:9317117-Interleukin-10,
pubmed-meshheading:9317117-Killer Cells, Natural,
pubmed-meshheading:9317117-Lymphoma,
pubmed-meshheading:9317117-Mast-Cell Sarcoma,
pubmed-meshheading:9317117-Mice,
pubmed-meshheading:9317117-Mice, Inbred C57BL,
pubmed-meshheading:9317117-Peptides,
pubmed-meshheading:9317117-Plasmacytoma,
pubmed-meshheading:9317117-Protein Processing, Post-Translational,
pubmed-meshheading:9317117-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:9317117-Transfection,
pubmed-meshheading:9317117-Tumor Cells, Cultured
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Down-regulation of the expression and function of the transporter associated with antigen processing in murine tumor cell lines expressing IL-10.
|
| pubmed:affiliation |
Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm, Sweden. flavio.salazar@mtc.ki.se
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|