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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1997-12-4
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pubmed:abstractText |
The Dictyostelium discoideum developmental program is initiated by starvation and its progress depends on G-protein-regulated transmembrane signaling. Disruption of the Dictyostelium G-protein alpha-subunit G alpha 3 (g alpha 3-) blocks development unless the mutant is starved in the presence of artificial cAMP pulses. The function of G alpha 3 was investigated by examining the expression of several components of the cAMP transmembrane signaling system in the g alpha 3- mutant. cAMP receptor 1 protein, cyclic nucleotide phosphodiesterase, phosphodiesterase inhibitor, and aggregation-stage adenylyl cyclase mRNA expression were absent or greatly reduced when cells were starved without exogenously applied pulses of cAMP. However, cAMP receptor 1 protein and aggregation-stage adenylyl cyclase mRNA expression were restored by starving the g alpha 3- cells in the presence of exogenous cAMP pulses. Adenylyl cyclase activity was also reduced in g alpha 3- cells starved without exogenous cAMP pulses compared with similarly treated wild-type cells but was elevated to a level twofold greater than wild-type cells in g alpha 3- cells starved in the presence of exogenous cAMP pulses. These results suggest that G alpha 3 is essential in early development because it controls the expression of components of the transmembrane signaling system.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-1315152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-1547939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-1670774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-1728597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-1836954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-1849108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-2049870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-2157967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-2536338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-2538821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-2993006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-3036630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-3072133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-3298997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-6092328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-6527688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-7556891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-7601348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-7612966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-7628643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-7774015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-7775572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-7790362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-7876207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-7925004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-8099335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-8110455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-8125261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9307965-8224844
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3',5'-Cyclic-AMP Phosphodiesterases,
http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/G protein a49B, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Protein alpha...,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Heterotrimeric GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/cyclic AMP receptor cAR1
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1059-1524
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1677-85
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9307965-3',5'-Cyclic-AMP Phosphodiesterases,
pubmed-meshheading:9307965-Adenylate Cyclase,
pubmed-meshheading:9307965-Animals,
pubmed-meshheading:9307965-Cell Line,
pubmed-meshheading:9307965-Cyclic AMP,
pubmed-meshheading:9307965-Dictyostelium,
pubmed-meshheading:9307965-Drosophila Proteins,
pubmed-meshheading:9307965-Enzyme Activation,
pubmed-meshheading:9307965-Fungal Proteins,
pubmed-meshheading:9307965-GTP-Binding Protein alpha Subunits, Gq-G11,
pubmed-meshheading:9307965-GTP-Binding Proteins,
pubmed-meshheading:9307965-Gene Expression Regulation, Developmental,
pubmed-meshheading:9307965-Gene Expression Regulation, Fungal,
pubmed-meshheading:9307965-Glycoproteins,
pubmed-meshheading:9307965-Heterotrimeric GTP-Binding Proteins,
pubmed-meshheading:9307965-Mutation,
pubmed-meshheading:9307965-RNA, Messenger,
pubmed-meshheading:9307965-Receptors, Cyclic AMP,
pubmed-meshheading:9307965-Signal Transduction
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pubmed:year |
1997
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pubmed:articleTitle |
G alpha 3 regulates the cAMP signaling system in Dictyostelium.
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pubmed:affiliation |
Department of Surgery, Wayne State University, Detroit, Michigan 48201, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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