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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1997-9-30
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pubmed:abstractText |
This study addresses the question of whether human peripheral CD4+ CD45RA+ T cells possess antigen-specific immune memory. CD4+ CD45RA+ T cells were isolated by a combination of positive and negative selection. Putative CD4+ CD45RA+ cells expressed CD45RA (98.9%) and contained < 0.1% CD4+ CD45RO+ and < 0.5% CD4+ CD45RA+ CD45RO+ cells. Putative CD45RO+ cells expressed CD45RO (90%) and contained 9% CD45RA+ CD45RO+ and < 0.1% CD4+ CD45RA+ cells. The responder frequency of Dermatophagoides pteronyssinus-stimulated CD4+ CD45RA+ and CD4+ CD45RO+ T cells was determined in two atopic donors and found to be 1:11,314 and 1:8031 for CD4+ CD45RA+ and 1:1463 and 1:1408 for CD4+ CD45RO+ T cells. The responder frequencies of CD4+ CD45RA+ and CD4+ CD45RO+ T cells from two non-atopic, but exposed, donors were 1:78031 and 1:176,903 for CD4+ CD45RA+ and 1:9136 and 1:13,136 for CD4+ CD45RO+ T cells. T cells specific for D. pteronyssinus were cloned at limiting dilution following 10 days of bulk culture with D. pteronyssinus antigen. Sixty-eight clones were obtained from CD4+ CD45RO+ and 24 from CD4+ CD45RA+ T cells. All clones were CD3+ CD4+ CD45RO+ and proliferated in response to D. pteronyssinus antigens. Of 40 clones tested, none responded to Tubercule bacillus purified protein derivative (PPD). No difference was seen in the pattern of interleukin-4 (IL-4) or interferon-gamma (IFN-gamma) producing clones derived from CD4+ CD45RA+ and CD4+ CD45RO+ precursors, although freshly isolated and polyclonally activated CD4+ CD45RA+ T cells produced 20-30-fold lower levels of IL-4 and IFN-gamma than their CD4+ CD45RO+ counterparts. Sixty per cent of the clones used the same pool of V beta genes. These data support the hypothesis that immune memory resides in CD4+ CD45RA+ as well as CD4+ CD45RO+ T cells during the chronic immune response to inhaled antigen.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-1345918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-1346105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-1355430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-1357074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-1436108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-1532395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-1671403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-1694200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-1834764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-1978253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-2191683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-2965180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-6488511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-7009746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-7085123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-7506722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-7512606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-7522154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-7615008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-7706706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-7790725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-7835932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-7910140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-7912547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-8064232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-8093440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-8149960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-8207196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-8287637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-8325322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-8340752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-8367485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-8476570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-8495489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9301520-9034154
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Allergens,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD45,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Dermatophagoides,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Variable Region,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
91
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
331-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9301520-Allergens,
pubmed-meshheading:9301520-Animals,
pubmed-meshheading:9301520-Antigens, CD45,
pubmed-meshheading:9301520-Antigens, Dermatophagoides,
pubmed-meshheading:9301520-CD4-Positive T-Lymphocytes,
pubmed-meshheading:9301520-Cell Culture Techniques,
pubmed-meshheading:9301520-Cell Differentiation,
pubmed-meshheading:9301520-Cell Division,
pubmed-meshheading:9301520-Clone Cells,
pubmed-meshheading:9301520-Epitopes,
pubmed-meshheading:9301520-Glycoproteins,
pubmed-meshheading:9301520-Humans,
pubmed-meshheading:9301520-Immunoglobulin Variable Region,
pubmed-meshheading:9301520-Immunologic Memory,
pubmed-meshheading:9301520-Mites,
pubmed-meshheading:9301520-Receptors, Antigen, T-Cell, alpha-beta
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pubmed:year |
1997
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pubmed:articleTitle |
Immune memory in CD4+ CD45RA+ T cells.
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pubmed:affiliation |
Department of Allergy and Respiratory Medicine, United Medical School of Guy's Hospital, London, UK.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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