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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1998-1-14
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pubmed:abstractText |
A simple method for the determination of the excitotoxin, quinolinic acid (QUIN) in cerebrospinal fluid (CSF) is described. QUIN, in lyophilized samples, was silylated by N-methyl-N-(tert.-butyldimethylsilyl)trifluoroacetamide in a single-step reaction at 65 to 70 degrees C to form a di-tert.-butyldimethylsilyl ester. Neither pre-purification of QUIN from CSF nor post-derivatisation sample clean-up was required. The derivatives were analysed by gas chromatography-electron impact mass spectrometry resulting in a prominent and characteristic [M-57]+ fragment ion which was used for quantitation. 2,6-Pyridine dicarboxylic acid, a structural analog of QUIN, was used as the internal standard. The detection limits for injected standards are in the femtomole range. CSF QUIN was found to be age-related and three preliminary reference ranges for CSF QUIN were found: 0 to 1 years, 31 +/- 15 nM QUIN (mean +/- standard deviation): 1.1 to 3 years, 26 +/- 15 nM; 3.1 to 14 years, 14 +/- 9 nM.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1387-2273
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
696
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
53-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9300908-Adolescent,
pubmed-meshheading:9300908-Age Factors,
pubmed-meshheading:9300908-Child,
pubmed-meshheading:9300908-Child, Preschool,
pubmed-meshheading:9300908-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:9300908-Humans,
pubmed-meshheading:9300908-Infant,
pubmed-meshheading:9300908-Infant, Newborn,
pubmed-meshheading:9300908-Quinolinic Acid,
pubmed-meshheading:9300908-Reference Standards,
pubmed-meshheading:9300908-Reference Values,
pubmed-meshheading:9300908-Reproducibility of Results,
pubmed-meshheading:9300908-Sensitivity and Specificity
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pubmed:year |
1997
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pubmed:articleTitle |
Concentrations of quinolinic acid in cerebrospinal fluid measured by gas chromatography and electron-impact ionisation mass spectrometry. Age-related changes in a paediatric reference population.
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pubmed:affiliation |
Division of Biochemistry and Genetics, Institute of Child Health (UCLMS), London, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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