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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
1997-9-30
|
| pubmed:abstractText |
Interleukin (IL)-4 is an immunomodulatory cytokine produced by a number of cell types including T cells, basophils, and mast cells. This pleiotropic cytokine has a number of immunoregulatory functions; however, the molecular mechanisms controlling the transcription of this gene are not yet completely understood. Several studies have implicated a possible autoregulatory mechanism for its own expression. Here, we have identified a Stat-6-responsive element (Stat-6RE) in the promoter of the human IL-4 gene. Utilizing electrophoretic mobility shift analysis, we have demonstrated the presence of two specific IL-4-responsive DNA-protein complexes in nuclear extracts of both human Th1 and Th2 clones. Phytohemagglutinin-blasted peripheral blood T cells also generated an inducible complex in response to stimulation with IL-4 and the IL-4-like cytokine IL-13. Transient transfection of the murine pre-B cell line BA/F3 stably transfected with the full-length human IL-4 receptor alpha chain demonstrated the ability of multicopy Stat-6RE to initiate transcription from a heterologous promoter upon IL-4 or IL-13 stimulation. These results indicate a possible autocrine mechanism for the regulation of IL-4 gene transcription through the Stat-6RE as well as a possible mechanism for IL-13 regulation of the human IL-4 promoter.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-13,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/STAT6 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/STAT6 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Stat6 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0014-2980
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
27
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1982-7
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9295035-Animals,
pubmed-meshheading:9295035-Base Sequence,
pubmed-meshheading:9295035-Cell Line,
pubmed-meshheading:9295035-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:9295035-Gene Expression Regulation,
pubmed-meshheading:9295035-Genes, Reporter,
pubmed-meshheading:9295035-Humans,
pubmed-meshheading:9295035-Interleukin-13,
pubmed-meshheading:9295035-Interleukin-4,
pubmed-meshheading:9295035-Mice,
pubmed-meshheading:9295035-Oligodeoxyribonucleotides,
pubmed-meshheading:9295035-Promoter Regions, Genetic,
pubmed-meshheading:9295035-STAT6 Transcription Factor,
pubmed-meshheading:9295035-Signal Transduction,
pubmed-meshheading:9295035-Th1 Cells,
pubmed-meshheading:9295035-Th2 Cells,
pubmed-meshheading:9295035-Trans-Activators,
pubmed-meshheading:9295035-Transfection
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Identification of a Stat-6-responsive element in the promoter of the human interleukin-4 gene.
|
| pubmed:affiliation |
Division of Basic Sciences, National Cancer Institute-Frederick Cancer Research and Development Center, USA.
|
| pubmed:publicationType |
Journal Article
|