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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1997-10-21
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pubmed:abstractText |
For a large number of T cell-mediated immunopathologies, the disease-related antigens are not yet identified. Identification of T cell epitopes is of crucial importance for the development of immune-intervention strategies. We show that CD4+ T cell epitopes can be defined by using a new system for synthesis and screening of synthetic peptide libraries. These libraries are designed to bind to the HLA class II restriction molecule of the CD4+ T cell clone of interest. The screening is based on three selection rounds using partial release of 14-mer peptides from synthesis beads and subsequent sequencing of the remaining peptide attached to the bead. With this approach, two peptides were identified that stimulate the beta cell-reactive CD4+ T cell clone 1c10, which was isolated from a newly diagnosed insulin-dependent diabetes mellitus patient. After performing amino acid-substitution studies and protein database searches, a Haemophilus influenzae TonB-derived peptide was identified that stimulates clone 1c10. The relevance of this finding for the pathogenesis of insulin-dependent diabetes mellitus is currently under investigation. We conclude that this system is capable of determining epitopes for (autoreactive) CD4+ T cell clones with previously unknown peptide specificity. This offers the possibility to define (auto)antigens by searching protein databases and/or to induce tolerance by using the peptide sequences identified. In addition the peptides might be used as leads to develop T cell receptor antagonists or anergy-inducing compounds.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-1546328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-1606619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-1709722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-1739971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-1944576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-2190098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-2191922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-2465903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-3679670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-7174209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-7477003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-7479792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-7522161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-7534214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-7629510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-7718741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-7772273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-7890324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-7969282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-8011285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-8265613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-8458693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-8464874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-8483498,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-8637859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-8691125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-8699011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-8700877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-8725947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-8729448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-8763818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294207-8772714
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
94
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10313-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1997
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pubmed:articleTitle |
The identification of CD4+ T cell epitopes with dedicated synthetic peptide libraries.
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pubmed:affiliation |
Department of Immunohematology and Blood Bank, Leiden University Hospital, University of Leiden, 2300 RC Leiden, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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