Linked Life Data

9290149

Source:http://linkedlifedata.com/resource/pubmed/id/9290149

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1997-10-15
pubmed:abstractText
This study characterizes the molecular mechanisms necessary for glutamine synthesis in the human placenta. RNA hybridization and protein immunoblotting were used to verify the presence of glutamine synthetase (GS) transcripts and protein, respectively. Additionally, the presence of GS was determined by immunohistochemistry. RNA hybridization demonstrated the presence of 1.8- and 2.8-kB transcripts and protein immunoblotting yielded a single 49-kDa band, characteristics of GS transcripts and protein, respectively. The mean (+/- s.d.) specific activity of placental GS, expressed as mumol gamma-glutamyl hydroxamic acid/mg protein/h was 1.80 +/- 0.59, which is comparable to other organs which are net glutamine producers. Immunohistochemical analysis indicated the presence of GS within the cytotrophoblast and mesenchyme layers of placental villi, but not in the syncytiotrophoblast. Although these results suggest that the human placenta is capable of synthesizing glutamine, the fate of glutamine produced by this organ remains speculative.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0143-4004
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
541-5
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Characterization of glutamine synthetase transcript, protein, and enzyme activity in the human placenta.
pubmed:affiliation
Department of Pediatrics, University of Florida College of Medicine, Gainesville 32610, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.