Linked Life Data

9286703

Source:http://linkedlifedata.com/resource/pubmed/id/9286703

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-11-18
pubmed:databankReference
pubmed:abstractText
The CSRP2 gene encoding the LIM domain protein CRP2 was originally identified in quail based on its strong transcriptional suppression in transformed avian fibroblasts. Here we have isolated a human CSRP2 cDNA clone encoding a 193-amino-acid human CRP2 (hCRP2) protein with 96.4% amino acid sequence identity to the avian homolog. The CSRP2 cDNA clone was used to isolate CSRP2-related clones from gamma EMBL3 and P1 libraries of human genomic DNA. The complete organization of the CSRP2 gene was determined by nucleic acid hybridization, transcriptional mapping, and nucleotide sequence analysis. The gene spans a total of approximately 22 kb and contains six exons. The coding region is confined to exons 2-6 and predicts a hCRP2 protein identical in its amino acid sequence to the protein deduced from the CSRP2 cDNA clone. By fluorescence in situ hybridization using both lambda EMBL3 and P1 library clones as hybridization probes and a new method for computerized signal localization, CSRP2 was mapped to chromosome subband 12q21.1, a region frequently affected by deletion or breakage events in various tumor types. The library screens also led to the isolation of a CSRP2-related pseudogene, CSRP2P, which carried several extensive deletions and nucleotide substitutions but no intervening sequences in comparison to the CSRP2 cDNA sequence. By physical linkage and fluorescence in situ hybridization, CSRP2P was mapped to chromosome subband 3q21.1.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0888-7543
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
83-93
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:9286703-Adaptor Proteins, Signal Transducing, pubmed-meshheading:9286703-Amino Acid Sequence, pubmed-meshheading:9286703-Animals, pubmed-meshheading:9286703-Avian Proteins, pubmed-meshheading:9286703-Base Sequence, pubmed-meshheading:9286703-Blotting, Northern, pubmed-meshheading:9286703-Blotting, Southern, pubmed-meshheading:9286703-Cells, Cultured, pubmed-meshheading:9286703-Chickens, pubmed-meshheading:9286703-Chromosome Mapping, pubmed-meshheading:9286703-Chromosomes, Human, Pair 12, pubmed-meshheading:9286703-Chromosomes, Human, Pair 3, pubmed-meshheading:9286703-Cloning, Molecular, pubmed-meshheading:9286703-Genetic Linkage, pubmed-meshheading:9286703-Humans, pubmed-meshheading:9286703-In Situ Hybridization, Fluorescence, pubmed-meshheading:9286703-LIM Domain Proteins, pubmed-meshheading:9286703-Mice, pubmed-meshheading:9286703-Molecular Sequence Data, pubmed-meshheading:9286703-Muscle Proteins, pubmed-meshheading:9286703-Nuclear Proteins, pubmed-meshheading:9286703-Proteins, pubmed-meshheading:9286703-Proto-Oncogene Proteins c-myc, pubmed-meshheading:9286703-Pseudogenes, pubmed-meshheading:9286703-Sequence Alignment, pubmed-meshheading:9286703-Sequence Analysis, DNA
pubmed:year
1997
pubmed:articleTitle
Cloning, structural analysis, and chromosomal localization of the human CSRP2 gene encoding the LIM domain protein CRP2.
pubmed:affiliation
Institute of Biochemistry, University of Innsbruck, Austria.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't