| pubmed-article:9274010 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9274010 | lifeskim:mentions | umls-concept:C0033809 | lld:lifeskim |
| pubmed-article:9274010 | lifeskim:mentions | umls-concept:C0935616 | lld:lifeskim |
| pubmed-article:9274010 | lifeskim:mentions | umls-concept:C0001511 | lld:lifeskim |
| pubmed-article:9274010 | lifeskim:mentions | umls-concept:C0005971 | lld:lifeskim |
| pubmed-article:9274010 | lifeskim:mentions | umls-concept:C0678695 | lld:lifeskim |
| pubmed-article:9274010 | lifeskim:mentions | umls-concept:C0806140 | lld:lifeskim |
| pubmed-article:9274010 | lifeskim:mentions | umls-concept:C0037110 | lld:lifeskim |
| pubmed-article:9274010 | lifeskim:mentions | umls-concept:C2347375 | lld:lifeskim |
| pubmed-article:9274010 | lifeskim:mentions | umls-concept:C2348042 | lld:lifeskim |
| pubmed-article:9274010 | lifeskim:mentions | umls-concept:C0150312 | lld:lifeskim |
| pubmed-article:9274010 | lifeskim:mentions | umls-concept:C1689985 | lld:lifeskim |
| pubmed-article:9274010 | pubmed:dateCreated | 1997-10-6 | lld:pubmed |
| pubmed-article:9274010 | pubmed:abstractText | The physico-chemical cell-surface properties of Pseudomonas aeruginosa AK1 and its adhesion to silicone rubber under flow were compared for cells suspended in phosphate-buffered saline (PBS) or PBS supplemented with 2% nutrient broth. Addition of 2% nutrient broth to cells suspended in PBS yielded minimal growth and did not significantly change the mean zeta potential of the organisms, which was around -13 mV. However, a comparatively larger proportion of the organisms had more negative zeta potentials in the presence of nutrient broth. This change was concurrent with a slight decrease in cell-surface hydrophobicity, as measured by water contact angles, from 119 degrees to around 112 degrees. The initial deposition rate of P. aeruginosa AK1 to silicone rubber, as studied in a parallel plate flow chamber, increased from 344 cm-2 s-1 in the absence of nutrient broth to 505 cm-2 s-1 in its presence. No stationary level of adhesion was observed in the presence of nutrient broth, instead the number of adhering cells increased steadily at a rate of approximately 85 cm-2 s-1. Fluorescent staining of adhering cells demonstrated that for adhesion from buffer only 2% of the adhering cells were metabolically active, whereas in case of deposition from PBS supplemented with nutrient broth, 67% of the adhering cells were metabolically active. It is concluded that the deposition rates measured in the parallel plate flow chamber with 2% nutrient broth added to the PBS suspension represent an interplay of adhesion and surface-associated growth. | lld:pubmed |
| pubmed-article:9274010 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9274010 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9274010 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9274010 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9274010 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9274010 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9274010 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9274010 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9274010 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9274010 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9274010 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9274010 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9274010 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9274010 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9274010 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9274010 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:9274010 | pubmed:issn | 1350-0872 | lld:pubmed |
| pubmed-article:9274010 | pubmed:author | pubmed-author:ReidGG | lld:pubmed |
| pubmed-article:9274010 | pubmed:author | pubmed-author:BusscherH JHJ | lld:pubmed |
| pubmed-article:9274010 | pubmed:author | pubmed-author:van der... | lld:pubmed |
| pubmed-article:9274010 | pubmed:author | pubmed-author:HabashM BMB | lld:pubmed |
| pubmed-article:9274010 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9274010 | pubmed:volume | 143 ( Pt 8) | lld:pubmed |
| pubmed-article:9274010 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9274010 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9274010 | pubmed:pagination | 2569-74 | lld:pubmed |
| pubmed-article:9274010 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:9274010 | pubmed:meshHeading | pubmed-meshheading:9274010-... | lld:pubmed |
| pubmed-article:9274010 | pubmed:meshHeading | pubmed-meshheading:9274010-... | lld:pubmed |
| pubmed-article:9274010 | pubmed:meshHeading | pubmed-meshheading:9274010-... | lld:pubmed |
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| pubmed-article:9274010 | pubmed:meshHeading | pubmed-meshheading:9274010-... | lld:pubmed |
| pubmed-article:9274010 | pubmed:meshHeading | pubmed-meshheading:9274010-... | lld:pubmed |
| pubmed-article:9274010 | pubmed:meshHeading | pubmed-meshheading:9274010-... | lld:pubmed |
| pubmed-article:9274010 | pubmed:meshHeading | pubmed-meshheading:9274010-... | lld:pubmed |
| pubmed-article:9274010 | pubmed:meshHeading | pubmed-meshheading:9274010-... | lld:pubmed |
| pubmed-article:9274010 | pubmed:meshHeading | pubmed-meshheading:9274010-... | lld:pubmed |
| pubmed-article:9274010 | pubmed:meshHeading | pubmed-meshheading:9274010-... | lld:pubmed |
| pubmed-article:9274010 | pubmed:meshHeading | pubmed-meshheading:9274010-... | lld:pubmed |
| pubmed-article:9274010 | pubmed:meshHeading | pubmed-meshheading:9274010-... | lld:pubmed |
| pubmed-article:9274010 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9274010 | pubmed:articleTitle | Adhesion of Pseudomonas aeruginosa to silicone rubber in a parallel plate flow chamber in the absence and presence of nutrient broth. | lld:pubmed |
| pubmed-article:9274010 | pubmed:affiliation | Department of Microbiology and Immunology, University of Western Ontario Health Sciences Centre, London, Canada. | lld:pubmed |
| pubmed-article:9274010 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9274010 | pubmed:publicationType | Comparative Study | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9274010 | lld:pubmed |
