Linked Life Data

9274010

Source:http://linkedlifedata.com/resource/pubmed/id/9274010

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1997-10-6
pubmed:abstractText
The physico-chemical cell-surface properties of Pseudomonas aeruginosa AK1 and its adhesion to silicone rubber under flow were compared for cells suspended in phosphate-buffered saline (PBS) or PBS supplemented with 2% nutrient broth. Addition of 2% nutrient broth to cells suspended in PBS yielded minimal growth and did not significantly change the mean zeta potential of the organisms, which was around -13 mV. However, a comparatively larger proportion of the organisms had more negative zeta potentials in the presence of nutrient broth. This change was concurrent with a slight decrease in cell-surface hydrophobicity, as measured by water contact angles, from 119 degrees to around 112 degrees. The initial deposition rate of P. aeruginosa AK1 to silicone rubber, as studied in a parallel plate flow chamber, increased from 344 cm-2 s-1 in the absence of nutrient broth to 505 cm-2 s-1 in its presence. No stationary level of adhesion was observed in the presence of nutrient broth, instead the number of adhering cells increased steadily at a rate of approximately 85 cm-2 s-1. Fluorescent staining of adhering cells demonstrated that for adhesion from buffer only 2% of the adhering cells were metabolically active, whereas in case of deposition from PBS supplemented with nutrient broth, 67% of the adhering cells were metabolically active. It is concluded that the deposition rates measured in the parallel plate flow chamber with 2% nutrient broth added to the PBS suspension represent an interplay of adhesion and surface-associated growth.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1350-0872
pubmed:author
pubmed:issnType
Print
pubmed:volume
143 ( Pt 8)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2569-74
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Adhesion of Pseudomonas aeruginosa to silicone rubber in a parallel plate flow chamber in the absence and presence of nutrient broth.
pubmed:affiliation
Department of Microbiology and Immunology, University of Western Ontario Health Sciences Centre, London, Canada.
pubmed:publicationType
Journal Article, Comparative Study