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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
1997-10-23
|
| pubmed:abstractText |
We determined if alveolar macrophages (AMs) from infants with severe recurrent wheezing episodes release increased amounts of tumour necrosis factor-alpha (TNF-alpha), as described in adults with asthma. We compared TNF-alpha release by unstimulated and lipopolysaccharide-stimulated AMs obtained by bronchoalveolar lavage in 13 wheezy and seven nonwheezy infants (aged 6-36 months) and analysed its regulation by dexamethasone. Metabolites in cell supernatants were quantified by enzyme-linked immunosorbent assay (ELISA) (TNF-alpha) or radioimmunoassay (thromboxane B2 and prostaglandin E2). Comparison of results was performed by the Mann-Whitney U-test and values were expressed as median (interquartile range) in ng x 10(6) cells(-1). Resting AMs from wheezy infants released larger amounts of TNF-alpha and thromboxane B2 as compared to controls: 2.67 (0.89-8.33) vs 0.48 (0.25-1.08) and 75.63 (38.07-158.91) vs 10.03 (7.36-76.08), respectively (p<0.05). When stimulated overnight with bacterial lipopolysaccharide, AMs from both groups released similar amounts of metabolites. Dexamethasone induced a consistent inhibition of the lipopolysaccharide-stimulated release of all the mediators. Our results show that alveolar macrophages from wheezy infants are activated to release increased amounts of tumour necrosis factor-alpha, as in asthma, and suggest that infants with recurrent wheezing may eventually benefit from treatment with glucocorticoids.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Dexamethasone,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Glucocorticoids,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Thromboxane B2,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0903-1936
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
10
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1767-73
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9272917-Bronchoalveolar Lavage Fluid,
pubmed-meshheading:9272917-Bronchoscopy,
pubmed-meshheading:9272917-Child, Preschool,
pubmed-meshheading:9272917-Dexamethasone,
pubmed-meshheading:9272917-Dinoprostone,
pubmed-meshheading:9272917-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:9272917-Female,
pubmed-meshheading:9272917-Glucocorticoids,
pubmed-meshheading:9272917-Humans,
pubmed-meshheading:9272917-Infant,
pubmed-meshheading:9272917-Lipopolysaccharides,
pubmed-meshheading:9272917-Macrophages, Alveolar,
pubmed-meshheading:9272917-Male,
pubmed-meshheading:9272917-Recurrence,
pubmed-meshheading:9272917-Reference Values,
pubmed-meshheading:9272917-Respiratory Sounds,
pubmed-meshheading:9272917-Thromboxane B2,
pubmed-meshheading:9272917-Tumor Necrosis Factor-alpha
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Increased spontaneous release of tumour necrosis factor-alpha by alveolar macrophages from wheezy infants.
|
| pubmed:affiliation |
Unité de Pharmacologie Cellulaire, Unité Associée Institut Pasteur-INSERM U-285, Paris, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|