9270030

Source:http://linkedlifedata.com/resource/pubmed/id/9270030

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0007621, umls-concept:C0028778, umls-concept:C0085828, umls-concept:C0243077, umls-concept:C0521447, umls-concept:C0871261, umls-concept:C1704632, umls-concept:C1706817, umls-concept:C1879547, umls-concept:C2911692
pubmed:issue	16
pubmed:dateCreated	1997-9-9
pubmed:abstractText	Cross-coupling of active protein-1 (AP-1) and nuclear factor (NF)-kappaB has been reported. In the present study, we investigated the possibility that both of these two transcription factors might contribute to the process of tumor promoter-induced transformation. To establish a stable reporter cell system, two reporter genes were stably transfected into a JB6 mouse tumor promotion-sensitive (P+) cell line: a luciferase reporter controlled by a collagenase AP-1 sequence and a chloramphenicol acetyltransferase reporter controlled by an interleukin 6 NF-kappaB sequence. This double-reporter cell line maintained the phenotype of tumor promotion sensitivity and was able to report basal or induced AP-1 and NF-kappaB transactivation. The cytokine tumor promoter tumor necrosis factor (TNF)-alpha transactivated NF-kappaB and AP-1 for both DNA binding and transcriptional activity. Pyrrolidine dithiocarbamate, an antioxidant that acts as an NF-kappaB inhibitor, efficiently inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA) or TNF-alpha induced NF-kappaB as well as AP-1 transactivation and cell transformation, suggesting dependency of transformation on both transcription factors. The AP-1 transrepressing-retinoid SR11302 transrepressed AP-1 and cell transformation when these were TPA induced but not when TNF-alpha induced, indicating different signaling pathways for TNF-alpha and TPA. Supershift electrophoresis mobility shift assay revealed that Jun B and c-Jun were absent from the AP-1/DNA complex following TNF-alpha but present following TPA treatment. Together, these results suggest that both AP-1 and NF-kappaB activation may be required for transformation whether induced by TPA or by TNF, and the differential sensitivity of TPA and TNF-alpha-induced transformation to inhibition by a retinoid might be explained by differences in the composition of the DNA-bound AP-1 complexes.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/2984705R
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Anticarcinogenic Agents, http://linkedlifedata.com/resource/pubmed/chemical/Carcinogens, http://linkedlifedata.com/resource/pubmed/chemical/DNA, http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B, http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidines, http://linkedlifedata.com/resource/pubmed/chemical/Retinoids, http://linkedlifedata.com/resource/pubmed/chemical/SR 11302, http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate, http://linkedlifedata.com/resource/pubmed/chemical/Thiocarbamates, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-1, http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha, http://linkedlifedata.com/resource/pubmed/chemical/pyrrolidine dithiocarbamic acid
pubmed:status	MEDLINE
pubmed:month	Aug
pubmed:issn	0008-5472
pubmed:author	pubmed-author:ColburnN HNH, pubmed-author:EieHH, pubmed-author:GhoseSS, pubmed-author:WestergaardCC
pubmed:issnType	Print
pubmed:day	15
pubmed:volume	57
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	3569-76
pubmed:dateRevised	2008-11-21
pubmed:meshHeading	pubmed-meshheading:9270030-Animals, pubmed-meshheading:9270030-Anticarcinogenic Agents, pubmed-meshheading:9270030-Carcinogens, pubmed-meshheading:9270030-Cell Transformation, Neoplastic, pubmed-meshheading:9270030-Cells, Cultured, pubmed-meshheading:9270030-DNA, pubmed-meshheading:9270030-Genes, Reporter, pubmed-meshheading:9270030-Mice, pubmed-meshheading:9270030-NF-kappa B, pubmed-meshheading:9270030-Pyrrolidines, pubmed-meshheading:9270030-Retinoids, pubmed-meshheading:9270030-Tetradecanoylphorbol Acetate, pubmed-meshheading:9270030-Thiocarbamates, pubmed-meshheading:9270030-Time Factors, pubmed-meshheading:9270030-Transcription Factor AP-1, pubmed-meshheading:9270030-Transcriptional Activation, pubmed-meshheading:9270030-Transfection, pubmed-meshheading:9270030-Tumor Necrosis Factor-alpha
pubmed:year	1997
pubmed:articleTitle	Inhibitors of both nuclear factor-kappaB and activator protein-1 activation block the neoplastic transformation response.
pubmed:affiliation	Laboratory of Biochemical Physiology, National Cancer Institute, Frederick Cancer Research and Development Center, NIH, Maryland 21702, USA. lij@ncifcrf.gov
pubmed:publicationType	Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't