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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
8
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pubmed:dateCreated |
1997-9-4
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pubmed:abstractText |
Immature B lineage acute lymphoblastic leukemia (ALL) is divided into two subtypes, 'pre-B' and 'early pre-B' ALL, by the presence or absence of cytoplasmic immunoglobulin (cIg). To study their clonal origin, we compared mu-chain transcripts in six cIg+ and eight cIg- ALL samples (CD10+/- CD19+ surface Ig-) with those in the normal phenotypic counterparts (CD10+ CD19+ surface Ig-) sorted from the bone marrow (BM). Northern blot analysis showed that the cIg+ ALL samples expressed greater amounts of mu-chain transcripts than the cIg- ALL samples. In the ALL samples and their counterparts, sequence analysis of mu-chain transcripts revealed infrequent somatic mutations of the V(H) genes and the similar usage of D and J(H) gene segments, but the length of complementarity determining region (CDR)-3 in the ALL samples was longer than that in the counterparts (50.0 +/- 15.5 vs 40.8 +/- 12.7 bp, P = 0.01). The mu-chain transcripts in the six cIg+ ALL samples and the counterparts (119/120 clones) had productive sequences, whereas those in the eight cIg- ALL samples had nonsense codons and/or frame shifts in their CDR-3. Our data suggest that a phenotype of ALL, 'pre-B' or 'early pre-B', is associated with V(H)-D-J(H) gene recombinatorial events, and that the CD10+ CD19+ surface Ig- population in the BM is not simply the cellular origin of ALL.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD19,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Variable Region,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin mu-Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Neprilysin,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0887-6924
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1274-80
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:9264381-Antigens, CD19,
pubmed-meshheading:9264381-B-Lymphocytes,
pubmed-meshheading:9264381-Base Sequence,
pubmed-meshheading:9264381-Bone Marrow,
pubmed-meshheading:9264381-Burkitt Lymphoma,
pubmed-meshheading:9264381-Cell Separation,
pubmed-meshheading:9264381-Child,
pubmed-meshheading:9264381-Child, Preschool,
pubmed-meshheading:9264381-Female,
pubmed-meshheading:9264381-Flow Cytometry,
pubmed-meshheading:9264381-Gene Rearrangement, B-Lymphocyte, Heavy Chain,
pubmed-meshheading:9264381-Genes, Immunoglobulin,
pubmed-meshheading:9264381-Hematopoiesis,
pubmed-meshheading:9264381-Humans,
pubmed-meshheading:9264381-Immunoglobulin Variable Region,
pubmed-meshheading:9264381-Immunoglobulin mu-Chains,
pubmed-meshheading:9264381-Male,
pubmed-meshheading:9264381-Molecular Sequence Data,
pubmed-meshheading:9264381-Neprilysin,
pubmed-meshheading:9264381-RNA, Messenger,
pubmed-meshheading:9264381-RNA, Neoplasm,
pubmed-meshheading:9264381-Transcription, Genetic
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pubmed:year |
1997
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pubmed:articleTitle |
Comparison of the immunoglobulin gene transcripts between immature B lineage acute lymphoblastic leukemia and the normal phenotypic counterparts in the bone marrow.
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pubmed:affiliation |
Department of Pediatrics, Nagoya University School of Medicine, Showa-ku, Japan.
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pubmed:publicationType |
Journal Article
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