Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1997-9-4
pubmed:abstractText
Immature B lineage acute lymphoblastic leukemia (ALL) is divided into two subtypes, 'pre-B' and 'early pre-B' ALL, by the presence or absence of cytoplasmic immunoglobulin (cIg). To study their clonal origin, we compared mu-chain transcripts in six cIg+ and eight cIg- ALL samples (CD10+/- CD19+ surface Ig-) with those in the normal phenotypic counterparts (CD10+ CD19+ surface Ig-) sorted from the bone marrow (BM). Northern blot analysis showed that the cIg+ ALL samples expressed greater amounts of mu-chain transcripts than the cIg- ALL samples. In the ALL samples and their counterparts, sequence analysis of mu-chain transcripts revealed infrequent somatic mutations of the V(H) genes and the similar usage of D and J(H) gene segments, but the length of complementarity determining region (CDR)-3 in the ALL samples was longer than that in the counterparts (50.0 +/- 15.5 vs 40.8 +/- 12.7 bp, P = 0.01). The mu-chain transcripts in the six cIg+ ALL samples and the counterparts (119/120 clones) had productive sequences, whereas those in the eight cIg- ALL samples had nonsense codons and/or frame shifts in their CDR-3. Our data suggest that a phenotype of ALL, 'pre-B' or 'early pre-B', is associated with V(H)-D-J(H) gene recombinatorial events, and that the CD10+ CD19+ surface Ig- population in the BM is not simply the cellular origin of ALL.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0887-6924
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1274-80
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:9264381-Antigens, CD19, pubmed-meshheading:9264381-B-Lymphocytes, pubmed-meshheading:9264381-Base Sequence, pubmed-meshheading:9264381-Bone Marrow, pubmed-meshheading:9264381-Burkitt Lymphoma, pubmed-meshheading:9264381-Cell Separation, pubmed-meshheading:9264381-Child, pubmed-meshheading:9264381-Child, Preschool, pubmed-meshheading:9264381-Female, pubmed-meshheading:9264381-Flow Cytometry, pubmed-meshheading:9264381-Gene Rearrangement, B-Lymphocyte, Heavy Chain, pubmed-meshheading:9264381-Genes, Immunoglobulin, pubmed-meshheading:9264381-Hematopoiesis, pubmed-meshheading:9264381-Humans, pubmed-meshheading:9264381-Immunoglobulin Variable Region, pubmed-meshheading:9264381-Immunoglobulin mu-Chains, pubmed-meshheading:9264381-Male, pubmed-meshheading:9264381-Molecular Sequence Data, pubmed-meshheading:9264381-Neprilysin, pubmed-meshheading:9264381-RNA, Messenger, pubmed-meshheading:9264381-RNA, Neoplasm, pubmed-meshheading:9264381-Transcription, Genetic
pubmed:year
1997
pubmed:articleTitle
Comparison of the immunoglobulin gene transcripts between immature B lineage acute lymphoblastic leukemia and the normal phenotypic counterparts in the bone marrow.
pubmed:affiliation
Department of Pediatrics, Nagoya University School of Medicine, Showa-ku, Japan.
pubmed:publicationType
Journal Article