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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1997-9-30
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pubmed:abstractText |
1. The present study was designed to test if microvascular coronary endothelial cells express ATP-sensitive K+ channels (KATP channels). We performed microfluorometric measurements of the membrane potential of freshly isolated guinea-pig coronary capillaries equilibrated with the voltage-sensitive dye bis-oxonol (bis-[1,3-dibutylbarbituric acid] trimethineoxonol, [DiBAC4(3)]). 2. The resting membrane potential of capillaries in physiological salt solution was -46 +/- 4.2 mV (n = 8) at room temperature (22 degrees C) as determined after calibration of the fluorescence using the Na(+)-K+ ionophore gramicidin in the presence of different K+ concentrations. Spontaneous membrane potential fluctuations of 10-20 mV amplitude were often observed. 3. A reversible, sustained hyperpolarization to a new membrane potential close to the K+ equilibrium potential (EK) could be induced by application of the K+ channel openers HOE 234 (100 nM to 1 microM), diazoxide (10 PM to 100 nM) or pinacidil (100 nM). Subsequent addition of glibenclamide (200 nM to 2 microM) reversed this hyperpolarization. 4. A glibenclamide-sensitive hyperpolarization of coronary capillaries to values near EK was also observed upon omission of D-glucose (10 mM) from the superfusing solution or by substituting L-glucose for D-glucose. Maximum hyperpolarization was reached in less than 10 min. 5. Our results suggest that microvascular coronary endothelial cells express KATP channels which may be activated during hypoglycaemia.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-1095722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-1542661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-1698266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-1895239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-2086764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-2315006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-2381766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-2443972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-2452599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-2458459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-2477294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-2621642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-2853223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-2871807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-6501412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-6704395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-7511348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-7522987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-7562603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-7928849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-7990720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-8006946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-8181103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-8186886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-8214038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-8331598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-8394257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-8494353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-8538499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-8591986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-8630239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-8889478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9263919-9019538
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Barbiturates,
http://linkedlifedata.com/resource/pubmed/chemical/Chromans,
http://linkedlifedata.com/resource/pubmed/chemical/Diazoxide,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Glyburide,
http://linkedlifedata.com/resource/pubmed/chemical/Gramicidin,
http://linkedlifedata.com/resource/pubmed/chemical/Guanidines,
http://linkedlifedata.com/resource/pubmed/chemical/HOE 234,
http://linkedlifedata.com/resource/pubmed/chemical/Isoxazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Pinacidil,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidines,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Vasodilator Agents,
http://linkedlifedata.com/resource/pubmed/chemical/bis(1,3-dibutylbarbiturate)trimethin...
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
502 ( Pt 2)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
397-408
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9263919-Animals,
pubmed-meshheading:9263919-Sodium Chloride,
pubmed-meshheading:9263919-Potassium,
pubmed-meshheading:9263919-Glucose,
pubmed-meshheading:9263919-Gramicidin,
pubmed-meshheading:9263919-Capillaries,
pubmed-meshheading:9263919-Guinea Pigs,
pubmed-meshheading:9263919-Barbiturates,
pubmed-meshheading:9263919-Fluorescent Dyes,
pubmed-meshheading:9263919-Guanidines,
pubmed-meshheading:9263919-Adenosine Triphosphate,
pubmed-meshheading:9263919-Chromans,
pubmed-meshheading:9263919-Vasodilator Agents,
pubmed-meshheading:9263919-Pyrrolidines,
pubmed-meshheading:9263919-Membrane Potentials,
pubmed-meshheading:9263919-Osmolar Concentration,
pubmed-meshheading:9263919-Diazoxide,
pubmed-meshheading:9263919-Coronary Circulation,
pubmed-meshheading:9263919-Muscle, Smooth, Vascular,
pubmed-meshheading:9263919-Glyburide,
pubmed-meshheading:9263919-Isoxazoles,
pubmed-meshheading:9263919-Pinacidil,
pubmed-meshheading:9263919-Potassium Channels
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