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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1980-2-26
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pubmed:abstractText |
Murine helper T cells activated to sheep or horse erythrocyte antigens in vivo have been established as continuous cell lines in culture. T cells require the presence of a T-cell growth factor (TCGF) for continuous proliferation. TCGF purified from murine, rat, or human sources all stimulate murine T-cell growth. The T-cell mitogens concanavalin A and phytohemagglutinin do not stimulate cell proliferation in continuous T-cell lines. All cells that grow in the presence of TCGF express Thy-1 antigens. Helper activity of T-cell lines is both antigen specific and effective for syngeneic or F1 B cells. Supernates from T-cell lines do not contain antigen-specific or nonspecific helper factors. Although several T-cell lines have shown stable helper activity for greater than 50 wk in culture, other cell lines have shown a gradual decline in effector function. The procedure used to establish and maintain proliferation of T cells in culture should be suitable for the selection and growth of antigen-specific effector T cells from each subclass.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/92524-107263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/92524-109511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/92524-145543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/92524-152789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/92524-181845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/92524-307029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/92524-308439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/92524-308989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/92524-310827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/92524-315987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/92524-4563349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/92524-69003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/92524-70355
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
150
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1510-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:92524-Animals,
pubmed-meshheading:92524-Antigens,
pubmed-meshheading:92524-Antigens, Surface,
pubmed-meshheading:92524-Cell Division,
pubmed-meshheading:92524-Cells, Cultured,
pubmed-meshheading:92524-Epitopes,
pubmed-meshheading:92524-Growth Substances,
pubmed-meshheading:92524-H-2 Antigens,
pubmed-meshheading:92524-Humans,
pubmed-meshheading:92524-Isoantigens,
pubmed-meshheading:92524-Lymphocyte Activation,
pubmed-meshheading:92524-Lymphocyte Cooperation,
pubmed-meshheading:92524-Mice,
pubmed-meshheading:92524-Mice, Inbred Strains,
pubmed-meshheading:92524-Rats,
pubmed-meshheading:92524-T-Lymphocytes
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pubmed:year |
1979
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pubmed:articleTitle |
Continuous proliferation of murine antigen-specific helper T lymphocytes in culture.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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