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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0007452,
umls-concept:C0010453,
umls-concept:C0010762,
umls-concept:C0010798,
umls-concept:C0020364,
umls-concept:C0023610,
umls-concept:C0033268,
umls-concept:C0035647,
umls-concept:C0036025,
umls-concept:C0043393,
umls-concept:C0162339,
umls-concept:C0439536,
umls-concept:C0534137,
umls-concept:C1413858
|
| pubmed:issue |
1
|
| pubmed:dateCreated |
1997-8-21
|
| pubmed:abstractText |
Bovine cytochrome P450c17 monooxygenase was produced in a 30-L fermenter by Saccharomyces cerevisiae GRF18 (YEp-Toku1), harboring the GAL10 promoter, and using conditions of medium cell density culture. Upon addition of D-galactose as an inducer and FeCl3 as a cofactor, cells began to produce the P450 hemoprotein. The yield of this enzyme reached a maximum after 31 h but its formation continued for more than 60 h after induction. The amount of P450c17 produced was 4.7-fold as compared to shake flask experiments.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
B
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0168-1656
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
23
|
| pubmed:volume |
56
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
57-61
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
1997
|
| pubmed:articleTitle |
Biotechnological potential of P450 monooxygenases high-level production of bovine cytochrome P450c17 monooxygenase during medium cell density culture of a recombinant yeast, Saccharomyces cerevisiae GRF 18 (YEp-Toku1).
|
| pubmed:affiliation |
Institute of Technical Biochemistry, University of Stuttgart, Germany.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|