Linked Life Data

9241046

Source:http://linkedlifedata.com/resource/pubmed/id/9241046

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-9-19
pubmed:abstractText
The apical and principal segments of the bovine acrosome contain a stable matrix complex that is bound to the outer acrosomal membrane and exhibits hydrolase-binding activity. The present study was undertaken to determine whether the outer acrosomal membrane-associated matrix complex (OMC) is composed of a unique set of acrosomal proteins and to define its fate during both capacitation and the acrosome reaction. A purified OMC fraction was isolated from ejaculated spermatozoa, and one polypeptide of 32 kDa (OMC32) was purified to homogeneity and used for N-terminal sequence analysis and preparation of monospecific antisera. Immunofluorescence staining of sperm with anti-OMC32 demonstrated that the polypeptide localized specifically to the apical and principal segments of the acrosome. Immunoelectron microscopy further revealed that OMC32 was restricted to the stable matrix assembly and was not associated with the inner acrosomal membrane or the equatorial segment. Immunoblot analyses of sperm lysates and of the purified OMC fraction revealed that anti-OMC32 recognized an antigenically related family of polypeptides between 38 and 19 kDa. These polypeptides exhibited no size processing during capacitation or the acrosome reaction, and they were not released during the acrosome reaction but remained in the particulate cell subfraction, associated with the hybrid membrane complex. N-terminal sequence analysis of OMC32 indicated a structural relationship to the SP-10 polypeptide family of human and baboon spermatozoa. The potential function of the OMC complex and differences in the intraacrosomal distribution of bovine OMC32-related polypeptides from that reported for acrosomal SP-10 polypeptides in other species are discussed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
325-34
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
An antigenically related polypeptide family is a major structural constituent of a stable acrosomal matrix assembly in bovine spermatozoa.
pubmed:affiliation
Department of Cell Biology, Vanderbilt University, Nashville, Tennessee 37232, USA. gary.olson@mcmail.vanderbilt.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.