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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1997-9-19
|
| pubmed:abstractText |
The gonadotrophins follicle stimulating hormone (FSH) and luteinizing hormone (LH) are key hormones in the regulation of ovarian function. In the present study, the expression of LH/human chorionic gonadotrophin (HCG) receptor mRNAs in the human ovary was examined. Northern blot analysis was used to measure relative amounts of LH/HCG receptor mRNA, and in-situ hybridization was used to localize LH/HCG receptor transcripts. Northern blot analysis of human ovaries detected three transcripts (5.4, 3.6 and 2.4 kb) for the LH/HCG receptor. LH/HCG receptor mRNA concentrations increased from preovulatory follicles to the corpus luteum of the midluteal phase, and decreased at the late luteal phase. Using in-situ hybridization, LH/HCG receptor mRNA was located predominantly in granulosa cells in the same follicle. Cloning of the human LH/HCG receptor cDNA previously revealed the existence of two alternative forms of the receptor differing by the presence (HLH-Ra) and absence (HLH-Rb) of 62 amino acids by exon 9. We have studied the functional significance of these receptor isoforms and have confirmed that they are generated by alternative splicing. A reverse transcription-polymerase chain reaction amplification was used to detect different isoforms of LH receptor mRNAs in ovary and placenta. The expression of the two mRNA forms of LH/HCG receptor were detected in ovary, and at very low concentrations in placenta. Treatment with HCG caused a dose-dependent increase in cAMP production with an initial response evident at approximately 1 ng/ml HCG in COS-7 cells expressing HLH-Ra. However, a complete loss of signal transduction was found in cells transfected with the truncated HLH-Rb.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chorionic Gonadotropin,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, LH
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
1360-9947
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
3
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
101-7
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9239715-Alternative Splicing,
pubmed-meshheading:9239715-Amino Acid Sequence,
pubmed-meshheading:9239715-Animals,
pubmed-meshheading:9239715-Base Sequence,
pubmed-meshheading:9239715-Blotting, Northern,
pubmed-meshheading:9239715-COS Cells,
pubmed-meshheading:9239715-Chorionic Gonadotropin,
pubmed-meshheading:9239715-Cyclic AMP,
pubmed-meshheading:9239715-DNA Primers,
pubmed-meshheading:9239715-Female,
pubmed-meshheading:9239715-Gene Expression,
pubmed-meshheading:9239715-Humans,
pubmed-meshheading:9239715-In Situ Hybridization,
pubmed-meshheading:9239715-Luteal Phase,
pubmed-meshheading:9239715-Molecular Sequence Data,
pubmed-meshheading:9239715-Ovary,
pubmed-meshheading:9239715-Placenta,
pubmed-meshheading:9239715-Polymerase Chain Reaction,
pubmed-meshheading:9239715-Pregnancy,
pubmed-meshheading:9239715-RNA, Messenger,
pubmed-meshheading:9239715-Receptors, LH,
pubmed-meshheading:9239715-Transfection
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Expression of luteinizing hormone/human chorionic gonadotrophin (LH/HCG) receptor mRNA in the human ovary.
|
| pubmed:affiliation |
Department of Obstetrics and Gynecology, Gunma University School of Medicine, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|