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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1997-9-4
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pubmed:abstractText |
A cDNA (1.6 kb) encoding a platelet protein receptor that binds type I collagen has been isolated from a human bone marrow cDNA library by using a degenerate oligonucleotide probe derived from the amino acid sequence of a CNBr fragment of the purified receptor. Computer search revealed that this cDNA represents the coding sequence of a unique protein. Using the prokaryotic expression system pKK 223-3-65 cDNA, a 54-kD recombinant protein was obtained and purified to apparent homogeneity. In an eukaryotic expression vector (pcDNA3-65 cDNA), a 65-kD protein was identified that was recognized by monoclonal anti-65 kD antibody (anti-65m). The recombinant protein binds to type I, but not to type III collagen by affinity column chromatography. The binding of the recombinant protein to type I collagen-coated Petri dishes is inhibited by anti-65m in a dose-dependent manner. The pcDNA3-65 cDNA-transfected nonadherent T cells express the protein, allowing them to attach to a type I collagen matrix, and are inhibited by anti-65m in a dose-dependent manner. Like the receptor protein purified from platelet membranes, the recombinant protein inhibits type I collagen-induced platelet aggregation and the adhesion of [14C]serotonin-labeled platelets to type I collagen in a dose-dependent manner. The recombinant protein neither binds to type III collagen-coated Petri dishes nor inhibits type III collagen and ADP-induced platelet aggregation, indicating specificity for type I collagen.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-1311144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-13871375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-14212418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-14285093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-1547344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-2156854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-2468670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-2545729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-2546619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-2808700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-282607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-2867998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-2941418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-3026823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-3598192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-3611052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-4009718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-5167575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-5820659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-6238974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-6330203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-6421343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-6466633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-7085640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-8134904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9239397-8180344
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
100
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
514-21
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9239397-Amino Acid Sequence,
pubmed-meshheading:9239397-Base Sequence,
pubmed-meshheading:9239397-Blood Platelets,
pubmed-meshheading:9239397-Cloning, Molecular,
pubmed-meshheading:9239397-Collagen,
pubmed-meshheading:9239397-Humans,
pubmed-meshheading:9239397-Integrins,
pubmed-meshheading:9239397-Molecular Sequence Data,
pubmed-meshheading:9239397-Receptors, Cell Surface,
pubmed-meshheading:9239397-Receptors, Collagen,
pubmed-meshheading:9239397-Sequence Analysis
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pubmed:year |
1997
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pubmed:articleTitle |
Cloning, characterization, and functional studies of a nonintegrin platelet receptor for type I collagen.
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pubmed:affiliation |
Department of Medicine, University of Tennessee at Memphis, Memphis, Tennessee 38104, USA. tchiang@utmem2.utmen.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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