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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1997-8-14
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pubmed:abstractText |
Antibodies to the sexual-stage surface antigens of Plasmodium falciparum, Pfs230 and Pfs48/45, can abolish the infectivity of gametes to mosquitoes; these antigens have been proposed as candidates for inclusion in a malaria transmission-blocking vaccine. One possible mechanism of antibody-mediated transmission blocking is complement-mediated gamete lysis. We have used a panel of human sera from geographically distinct regions where malaria is endemic to investigate whether this may be a mechanism of naturally acquired transmission-blocking immunity to P. falciparum. By immunoprecipitation, we have shown that antibody recognition of Pfs230 and Pfs48/45 is limited, despite universal exposure to P. falciparum gametocytes. In vitro complement-mediated lysis of P. falciparum gametes was positively associated with the presence of antibodies to Pfs230 but not with antibodies to the N-terminal region of the precursor molecule (Pfs260), which is shed from the gametocyte surface at the time of gametogenesis. Similarly, antibodies to two other gametocyte-specific proteins, Pfs48/45 and Pfg27/25, were not associated with gamete lysis. All sera which mediate gamete lysis contain immunoglobulin G1 (IgG1) and/or IgG3 antibodies to gamete surface proteins as determined by an enzyme-linked immunosorbent assay. These data suggest that Pfs230 is a major target of complement-fixing antibodies which may be important for antibody-mediated transmission-blocking immunity.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-1707506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-1719534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-1721820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-2112574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-2422080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-2447164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-2459062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-2642527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-2865324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-2867467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-3069711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-3287282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-3299700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-3318021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-3500259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-3528848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-37101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-3899923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-6257680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-6350527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-6396517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-6631012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-7031476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-7086709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-7310746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-7529717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-7532850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-7591074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-7822011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-7856826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-7908734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-8015855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-8058360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-8220736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-8479460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-8720173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234748-8813686
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
65
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3017-23
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9234748-Adolescent,
pubmed-meshheading:9234748-Adult,
pubmed-meshheading:9234748-Aged,
pubmed-meshheading:9234748-Animals,
pubmed-meshheading:9234748-Antibodies, Protozoan,
pubmed-meshheading:9234748-Child,
pubmed-meshheading:9234748-Child, Preschool,
pubmed-meshheading:9234748-Complement System Proteins,
pubmed-meshheading:9234748-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:9234748-Humans,
pubmed-meshheading:9234748-Immune Sera,
pubmed-meshheading:9234748-Immunoglobulin G,
pubmed-meshheading:9234748-Infant,
pubmed-meshheading:9234748-Middle Aged,
pubmed-meshheading:9234748-Plasmodium falciparum,
pubmed-meshheading:9234748-Precipitin Tests
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pubmed:year |
1997
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pubmed:articleTitle |
Complement-mediated lysis of Plasmodium falciparum gametes by malaria-immune human sera is associated with antibodies to the gamete surface antigen Pfs230.
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pubmed:affiliation |
Institute of Cell, Animal and Population Biology, Division of Biological Sciences, University of Edinburgh, Scotland, United Kingdom. Julie.healer@ed.ac.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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