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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1997-10-16
|
| pubmed:abstractText |
We studied peripheral blood and apheresis samples from 39 consecutive normal donors who were parents or siblings of patients who received matched or mismatched bone marrow transplants using a combination of rhG-CSF-mobilized peripheral blood stem cells (PBSCs) and bone marrow (BM). BM was harvested from donors 1-7 days before starting rhG-CSF treatment: 12 micrograms/kg/day rhG-CSF was administered by continuous s.c. infusion for 4-7 days. Peripheral blood progenitor cells were harvested by leukapheresis using an automated continuous-flow blood cell separator, beginning on day 4 of rhG/CSF, for 1-4 consecutive days. Peak peripheral blood CD34+ cell and CFU-GM levels were reached simultaneously on day 5 or 6 of rhG-CSF administration. Median peak levels were 1.65% for CD34+ cells (range 0.34%-4.7%) and 142 CFU-GM/10(5) plated cells (range 16-700). The greatest numbers of CD34+ cells and CFU-GM, expressed per liter of blood volume processed, were harvested during the second and third leukapheresis: CD34+ cells 37.77 +/- 25.48 x 10(6) and CFU-GM 3.32 +/- 2.51 x 10(6) during the second leukapheresis, and CD34+ cells 37.01 +/- 16.33 x 10(6) and CFU-GM 3.82 +/- 4.36 x 10(6) during the third. The number of CD34+ cells and CFU-GM did not correlate with the sex, age, or body weight of the donors. This study indicates that this protocol for administration of rhG-CSF mobilizes large numbers of hematopoietic progenitor cells into the peripheral blood and that bone marrow harvesting before G-CSF administration does not impair stem cell mobilization.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
1061-6128
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
6
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
227-34
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9234177-Adult,
pubmed-meshheading:9234177-Aged,
pubmed-meshheading:9234177-Bone Marrow Cells,
pubmed-meshheading:9234177-Cell Movement,
pubmed-meshheading:9234177-Cell Separation,
pubmed-meshheading:9234177-Female,
pubmed-meshheading:9234177-Follow-Up Studies,
pubmed-meshheading:9234177-Granulocyte Colony-Stimulating Factor,
pubmed-meshheading:9234177-Granulocytes,
pubmed-meshheading:9234177-Hematopoietic Stem Cells,
pubmed-meshheading:9234177-Humans,
pubmed-meshheading:9234177-Immunophenotyping,
pubmed-meshheading:9234177-Leukapheresis,
pubmed-meshheading:9234177-Leukocytes, Mononuclear,
pubmed-meshheading:9234177-Macrophages,
pubmed-meshheading:9234177-Male,
pubmed-meshheading:9234177-Middle Aged,
pubmed-meshheading:9234177-Tissue Donors
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Stem cell mobilization in normal donors.
|
| pubmed:affiliation |
Department of Clinical Medicine, Pathology, and Pharmacology, Haematology, University of Perugia, Italy.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|