|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
7
|
|
pubmed:dateCreated |
1997-9-29
|
|
pubmed:abstractText |
Rat liver recombinant BR1UGT1.1 was found to have significant activity toward retinoid substrates. UGT1.1 glucuronidation activity was 91 +/- 18 pmol/mg x min for atRA and 113 +/- 19 pmol/mg x min for 5,6-epoxy-atRA. The apparent K(M) and V(max) of atRA acid glucuronidation by UGT1.1 were 59.1 +/- 5.4 microM and 158 +/- 43 pmol/mg x min, respectively. SDS-PAGE and Western blot analysis of UGT1.1-transfected HK293 membrane proteins photolabeled with [11,12-3H]atRA revealed a protein of approximately 56 kDa that was labeled by [3H]atRA, detected by anti-pNP UGT antibody and not present in membranes from nontransfected HK293 cells. Liver microsomes from Gunn rats, which lack UGT1.1, had significant activity toward atRA (111 +/- 28 pmol/mg x min).
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
0090-9556
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
25
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
889-92
|
|
pubmed:dateRevised |
2007-11-14
|
|
pubmed:meshHeading |
pubmed-meshheading:9224784-Affinity Labels,
pubmed-meshheading:9224784-Animals,
pubmed-meshheading:9224784-Blotting, Western,
pubmed-meshheading:9224784-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9224784-Glucuronates,
pubmed-meshheading:9224784-Glucuronosyltransferase,
pubmed-meshheading:9224784-Humans,
pubmed-meshheading:9224784-Isoenzymes,
pubmed-meshheading:9224784-Kinetics,
pubmed-meshheading:9224784-Microsomes, Liver,
pubmed-meshheading:9224784-Precipitin Tests,
pubmed-meshheading:9224784-Rats,
pubmed-meshheading:9224784-Rats, Gunn,
pubmed-meshheading:9224784-Rats, Sprague-Dawley,
pubmed-meshheading:9224784-Rats, Wistar,
pubmed-meshheading:9224784-Recombinant Proteins,
pubmed-meshheading:9224784-Retinoids,
pubmed-meshheading:9224784-Substrate Specificity
|
|
pubmed:year |
1997
|
|
pubmed:articleTitle |
Glucuronidation of retinoids by rat recombinant UDP: glucuronosyltransferase 1.1 (bilirubin UGT).
|
|
pubmed:affiliation |
Department of Medicine, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
|
