Linked Life Data

9212793

Source:http://linkedlifedata.com/resource/pubmed/id/9212793

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1997-7-28
pubmed:abstractText
The white-ivory assay of Drosophila is based on the detection of reversions to wild-type phenotype of ommatidia with the white-ivory mutation. A tandem quadruplication of this gene is used in order to increase the reversion probability. Although the exact mechanism implicated in reversion is not known, revertant spots are believed to arise as a consequence of intrachromosmal recombination or related phenomena. Since the white-ivory assay has not been broadly used, the number of chemicals tested until now is still limited. In this work, we have assayed 25 chemicals belonging to several chemical groups, i.e., crosslinking agents, DNA-topoisomerase inhibitors, antimetabolites/nucleotide pool inhibitors, cyclic-adduct inducers, halogenated hydrocarbons, bulky-adduct inducers, intercalating agents, oxidative damage inducers, and a multiple damage inducer, to validate this test. Cross-linking agents, halogenated hydrocarbons, and the multiple damage inducer, dounomycin, were positive. On the contrary, the three antimetabolites/nucleotide pool inhibitors tested were negative. The other chemical groups showed disparate results, since some chemicals were positive, whereas others were negative in each group. A comparison with the results obtained in the w/ w+ and mwh/flr3 assays shows that the wi assay detects a more restricted spectrum of damages than those, although, with respect to carcinogenicity, its sensitivity (0.76, with the 62 chemicals tested until now) is similar to that estimated for the mentioned somatic assays. The conclusion of this work, then, is that the wi assay is not recommended as a general screening test, because the background reversion frequencies show a high variability among solvents, the range of lesion-recognition is lower than in the w/ w+ and mwh/flr3 SMARTs, and the mechanism implicated in the white-ivory reversion is poorly understood.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0893-6692
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
406-17
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:9212793-Acetaldehyde, pubmed-meshheading:9212793-Animals, pubmed-meshheading:9212793-Antimetabolites, pubmed-meshheading:9212793-Cross-Linking Reagents, pubmed-meshheading:9212793-DNA Adducts, pubmed-meshheading:9212793-DNA Damage, pubmed-meshheading:9212793-Drosophila melanogaster, pubmed-meshheading:9212793-Enzyme Inhibitors, pubmed-meshheading:9212793-Evaluation Studies as Topic, pubmed-meshheading:9212793-Eye Color, pubmed-meshheading:9212793-Hydrocarbons, Halogenated, pubmed-meshheading:9212793-Intercalating Agents, pubmed-meshheading:9212793-Male, pubmed-meshheading:9212793-Mutagenicity Tests, pubmed-meshheading:9212793-Oxidative Stress, pubmed-meshheading:9212793-Recombination, Genetic, pubmed-meshheading:9212793-Sensitivity and Specificity, pubmed-meshheading:9212793-Topoisomerase I Inhibitors, pubmed-meshheading:9212793-Topoisomerase II Inhibitors
pubmed:year
1997
pubmed:articleTitle
Is the white-ivory assay of Drosophila melanogaster a useful tool in genetic toxicology?
pubmed:affiliation
Departamento de Biología Funcional, Universidad de Oviedo, Spain.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't