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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1997-7-28
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pubmed:databankReference |
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pubmed:abstractText |
Three lambda phage clones encompassing the Na+/phosphate co-transporter (NaPi-3) gene and its 5' flanking region were isolated from a human genomic DNA library. The gene comprises 13 exons and 12 introns and spans approx. 14 kb. All exon-intron junctions conform to the GT/AG rule. The major transcription-initiation site was determined by primer-extension analysis and is an adenosine residue 57 bp upstream of the 3' end of the first exon. There is a typical TATA box 28 bp upstream of the major transcription-initiation site and various cis-acting elements, including a cAMP-responsive element, AP-1, AP-2 and SP-1 sites in the 5' flanking region. This region also contains three direct-repeat-like sequences that resemble the consensus binding sequence for members of the steroid-thyroid hormone receptor superfamily, including vitamin D. Deletion analysis suggests that the region from nt-2409 to nt-1259 in the 5' flanking region may be involved in kidney-specific gene expression. Vitamin D responsiveness of the NaPi-3 promoter was also detected in COS-7 cells co-transfected with a human vitamin D receptor expression vector. The presence of the three vitamin D receptor- responsive elements in the NaPi-3 promoter may be important in mediating the enhanced expression of the gene by 1,25-dihydroxyvitamin D3.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-1372904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-1601885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-1648450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-1740408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-1946375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-2020552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-2827008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-3342065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-3790614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-3839137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-4058579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-6331201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-6791577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-6897631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-7509808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-7518962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-7570593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-7611445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-7729529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-7733319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-7826357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-7929070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-7977794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-8070635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-8113402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-8120016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-8195156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-8327470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-8567640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-8687373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-8691748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-8693007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210418-8867793
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
324 ( Pt 3)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
927-34
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9210418-Amino Acid Sequence,
pubmed-meshheading:9210418-Animals,
pubmed-meshheading:9210418-Base Sequence,
pubmed-meshheading:9210418-COS Cells,
pubmed-meshheading:9210418-Calcitriol,
pubmed-meshheading:9210418-Carrier Proteins,
pubmed-meshheading:9210418-DNA, Complementary,
pubmed-meshheading:9210418-HeLa Cells,
pubmed-meshheading:9210418-Humans,
pubmed-meshheading:9210418-Molecular Sequence Data,
pubmed-meshheading:9210418-RNA Splicing,
pubmed-meshheading:9210418-Sodium-Phosphate Cotransporter Proteins,
pubmed-meshheading:9210418-Symporters,
pubmed-meshheading:9210418-Transcription, Genetic,
pubmed-meshheading:9210418-Vitamin D
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pubmed:year |
1997
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pubmed:articleTitle |
Gene structure and functional analysis of the human Na+/phosphate co-transporter.
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pubmed:affiliation |
Department of Clinical Nutrition, School of Medicine, University of Tokushima, Kuramoto-cho 3, Tokushima 770, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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