Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1997-7-24
pubmed:abstractText
Fluoride is an acknowledged bone-forming agent that may act through stimulation of osteoblast proliferation. Fluoride's action on osteoblasts and bone is potentiated by aluminum, which can form a complex with fluoride (fluoroaluminate) and activate heterotrimeric G proteins. Here we examined signaling pathways activated by fluoroaluminate in MC3T3-E1 osteoblastic and in NIH3T3 fibroblastic cells. In MC3T3-E1 cells, fluoroaluminate induced a decrease in cAMP levels and an increase in MAP and p70 S6 kinase phosphorylations. These responses were partially or completely prevented by pertussis toxin, an inhibitor of G alpha i proteins. In NIH3T3 cells, fluoroaluminate induced weaker tyrosine and MAP kinase phosphorylations. Fluoroaluminate, but not PDGF, induced a long-lasting tyrosine phosphorylation of a 130 kDa protein only in MC3T3-E1 cells. The expression of G alpha i2, but not of G alpha s and G alpha q/11 proteins was about 10-fold higher in MC3T3-E1 cells. Thus, different signaling in osteoblastic and fibroblastic cells may be due to differential expression of G alpha i proteins and tyrosine kinase substrates and could underlie fluoride's pharmacological action in bone.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
235
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
680-4
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:9207219-3T3 Cells, pubmed-meshheading:9207219-Aluminum, pubmed-meshheading:9207219-Animals, pubmed-meshheading:9207219-Calcium-Calmodulin-Dependent Protein Kinases, pubmed-meshheading:9207219-Cyclic AMP, pubmed-meshheading:9207219-Fibroblasts, pubmed-meshheading:9207219-Fluorine, pubmed-meshheading:9207219-Forskolin, pubmed-meshheading:9207219-Kinetics, pubmed-meshheading:9207219-Mice, pubmed-meshheading:9207219-Osteoblasts, pubmed-meshheading:9207219-Pertussis Toxin, pubmed-meshheading:9207219-Phosphorylation, pubmed-meshheading:9207219-Phosphotyrosine, pubmed-meshheading:9207219-Protein-Serine-Threonine Kinases, pubmed-meshheading:9207219-Ribosomal Protein S6 Kinases, pubmed-meshheading:9207219-Signal Transduction, pubmed-meshheading:9207219-Virulence Factors, Bordetella
pubmed:year
1997
pubmed:articleTitle
Fluoroaluminate induces pertussis toxin-sensitive protein phosphorylation: differences in MC3T3-E1 osteoblastic and NIH3T3 fibroblastic cells.
pubmed:affiliation
Novartis Pharma AG, Basel, Switzerland. susami1@fmi.ch
pubmed:publicationType
Journal Article