|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
14
|
|
pubmed:dateCreated |
1997-8-5
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
The prolamin box (P-box) is a highly conserved 7-bp sequence element (5'-TGTAAAG-3') found in the promoters of many cereal seed storage protein genes. Nuclear factors from maize endosperm specifically interact with the P-box present in maize prolamin genes (zeins). The presence of the P-box in all zein gene promoters suggests that interactions between endosperm DNA binding proteins and the P-box may play an important role in the coordinate activation of zein gene expression during endosperm development. We have cloned an endosperm-specific maize cDNA, named prolamin-box binding factor (PBF), that encodes a member of the recently described Dof class of plant Cys2-Cys2 zinc-finger DNA binding proteins. When tested in gel shift assays, PBF exhibits the same sequence-specific binding to the P-box as factors present in maize endosperm nuclei. Additionally, PBF interacts in vitro with the basic leucine zipper protein Opaque2, a known transcriptional activator of zein gene expression whose target site lies 20 bp downstream of the P-box in the 22-kDa zein gene promoter. The isolation of the PBF gene provides an essential tool to further investigate the functional role of the highly conserved P-box in regulating cereal storage protein gene expression.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-1392590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-1392591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-1538693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-15981330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-1899843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-2120592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-2296602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-2370845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-2479535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-2535458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-2658986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-3025847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-4059057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-7567449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-7696880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-7766871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-8007944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-8220485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-8242750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-8340424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-8440244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-8453304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-8718629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-8771779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-8929416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-9011080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207153-9061949
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
8
|
|
pubmed:volume |
94
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
7685-90
|
|
pubmed:dateRevised |
2010-9-13
|
|
pubmed:meshHeading |
pubmed-meshheading:9207153-Amino Acid Sequence,
pubmed-meshheading:9207153-DNA-Binding Proteins,
pubmed-meshheading:9207153-Leucine Zippers,
pubmed-meshheading:9207153-Molecular Sequence Data,
pubmed-meshheading:9207153-Plant Proteins,
pubmed-meshheading:9207153-Promoter Regions, Genetic,
pubmed-meshheading:9207153-Sequence Alignment,
pubmed-meshheading:9207153-Transcription Factors,
pubmed-meshheading:9207153-Zea mays,
pubmed-meshheading:9207153-Zinc Fingers
|
|
pubmed:year |
1997
|
|
pubmed:articleTitle |
A maize zinc-finger protein binds the prolamin box in zein gene promoters and interacts with the basic leucine zipper transcriptional activator Opaque2.
|
|
pubmed:affiliation |
Department of Biology, University of California at San Diego, Mail Code 0116, La Jolla, CA 92093, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
