Linked Life Data

9201219

Source:http://linkedlifedata.com/resource/pubmed/id/9201219

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-7-24
pubmed:abstractText
Recent studies have demonstrated biased usage of TCR V beta 17 and a high degree of diversity in J beta usage within the influenza virus matrix epitope (M.58-66)-specific CTL response. In contrast, in the course of a study on the cellular response to influenza A virus, we found preferential usage of V beta 17-J beta 2.2 rearrangement in an individual with an unexpectedly high number of CTL precursors (CTLp). We took advantage of such situation to study the longitudinal repertoire of the CD8+ T cell precursors. By limiting dilution analysis combined with the use of a clonotypic primer corresponding to the CDR3 region of this matrix-specific TCR V beta chain, the influenza-specific CTLp were shown to be stable for a period of 6 years. Overall, our results show that virus-specific CTLp can be directly monitored in vivo by molecular fingerprinting without in vitro restimulation. These findings might be extremely important for evaluation of the specific immune response to a given human pathogen.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
233
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
93-104
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9201219-Animals, pubmed-meshheading:9201219-Antigens, Viral, pubmed-meshheading:9201219-Cells, Cultured, pubmed-meshheading:9201219-Chick Embryo, pubmed-meshheading:9201219-Cloning, Molecular, pubmed-meshheading:9201219-Cytotoxicity Tests, Immunologic, pubmed-meshheading:9201219-Humans, pubmed-meshheading:9201219-Influenza A virus, pubmed-meshheading:9201219-Leukocytes, Mononuclear, pubmed-meshheading:9201219-Longitudinal Studies, pubmed-meshheading:9201219-Nucleoproteins, pubmed-meshheading:9201219-Peptides, pubmed-meshheading:9201219-RNA-Binding Proteins, pubmed-meshheading:9201219-Receptors, Antigen, T-Cell, alpha-beta, pubmed-meshheading:9201219-T-Lymphocytes, Cytotoxic, pubmed-meshheading:9201219-Viral Core Proteins, pubmed-meshheading:9201219-Viral Matrix Proteins
pubmed:year
1997
pubmed:articleTitle
In vivo longitudinal analysis of a dominant TCR repertoire selected in human response to influenza virus.
pubmed:affiliation
Institut National de la Santé et de la Recharche Médicale, Unit 445, Paris, France. armelle.prevost_blondel@icgm.cochin.inserm.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't