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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1997-8-5
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pubmed:abstractText |
The production of alloreactive cytotoxic T-lymphocytes (CTL) for therapy of recurrent brain tumours was performed in the CELLMAX artificial capillary system composed of cell-culture modules containing cellulose acetate or cuprammonium rayon hollow fibres. Lymphocytes, obtained from the brain-tumour patient to be used for sensitization, were stimulated with OKT3 (anti-CD3) and interleukin-2 (IL-2) and inoculated into the extracapillary space of artificial capillary modules. For allogeneic CTL production, the expanded patient lymphocytes were harvested, irradiated and placed into a second artificial capillary system with allogeneic lymphocytes from a healthy donor. In a one-way mixed lymphocyte reaction, CTL developed in the presence of low-concentration IL-2 (60 i.u. of IL-2/ml). In 18-21 days the cell preparation usually displayed a predominantly CD3+, CD8+ phenotype, which consorted with the dual-labelled CD8/CD11a markers used to identify CTL. Chromium (Cr)-release assays demonstrated lysis of patient tumour in relation to allogeneic glioma; the response observed in cold-target-inhibition assays confirmed lysis of the relevant tumour by the CTL.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AM 160H,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD8,
http://linkedlifedata.com/resource/pubmed/chemical/Biocompatible Materials,
http://linkedlifedata.com/resource/pubmed/chemical/Cellulose,
http://linkedlifedata.com/resource/pubmed/chemical/Chromium,
http://linkedlifedata.com/resource/pubmed/chemical/Immunosuppressive Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphocyte Function-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Membranes, Artificial,
http://linkedlifedata.com/resource/pubmed/chemical/Muromonab-CD3,
http://linkedlifedata.com/resource/pubmed/chemical/acetylcellulose
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0885-4513
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
25 ( Pt 3)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
197-205
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9198273-Antibodies, Monoclonal,
pubmed-meshheading:9198273-Antigens, CD3,
pubmed-meshheading:9198273-Antigens, CD8,
pubmed-meshheading:9198273-Biocompatible Materials,
pubmed-meshheading:9198273-Brain Neoplasms,
pubmed-meshheading:9198273-Cells, Cultured,
pubmed-meshheading:9198273-Cellulose,
pubmed-meshheading:9198273-Chromium,
pubmed-meshheading:9198273-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:9198273-Glioma,
pubmed-meshheading:9198273-Humans,
pubmed-meshheading:9198273-Immunosuppressive Agents,
pubmed-meshheading:9198273-Immunotherapy, Adoptive,
pubmed-meshheading:9198273-Interleukin-2,
pubmed-meshheading:9198273-Lymphocyte Activation,
pubmed-meshheading:9198273-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:9198273-Lymphocyte Function-Associated Antigen-1,
pubmed-meshheading:9198273-Membranes, Artificial,
pubmed-meshheading:9198273-Muromonab-CD3,
pubmed-meshheading:9198273-Neoplasm Recurrence, Local,
pubmed-meshheading:9198273-Phenotype,
pubmed-meshheading:9198273-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:9198273-Tumor Cells, Cultured
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pubmed:year |
1997
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pubmed:articleTitle |
Artificial-capillary-system development of human alloreactive cytotoxic T-lymphocytes that lyse brain tumours.
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pubmed:affiliation |
Department of Immunology, University of Colorado Health Sciences Center, Denver 80262, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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