Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-7-15
pubmed:databankReference
pubmed:abstractText
Measles virus nucleoprotein encoded from the vaccinia virus genome assembles into nucleocapsids similar in many respects to those observed during a natural measles virus infection. The influence of the measles virus phosphoprotein on nucleocapsid assembly has been studied using a vaccinia virus recombinant encoding both the nucleoprotein and the phosphoprotein. Infection of cells with the virus recombinant resulted in the formation of cytoplasmic inclusions in which the nucleoprotein and the phosphoprotein colocalized. Electron microscopic examination suggested that these inclusions contained characteristic nucleocapsid filaments. The buoyant density of nucleocapsids assembled in the presence of the phosphoprotein was found to be slightly higher than that of nucleocapsids assembled in its absence. Furthermore, the phosphoprotein partially inhibited the formation of nucleocapsids, a process which was extremely efficient when the nucleoprotein was expressed alone. Analysis of the nucleic acid content of nucleocapsids showed that they packaged heterologous RNA into a micrococcal nuclease-resistant form. These experiments demonstrate that the measles virus phosphoprotein regulates the efficiency with which the nucleoprotein assembles into nucleocapsids and the structural conformation they acquire.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:day
9
pubmed:volume
232
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
260-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9191839-Animals, pubmed-meshheading:9191839-Base Sequence, pubmed-meshheading:9191839-Cell Line, pubmed-meshheading:9191839-Centrifugation, Density Gradient, pubmed-meshheading:9191839-Cercopithecus aethiops, pubmed-meshheading:9191839-Cricetinae, pubmed-meshheading:9191839-DNA, Viral, pubmed-meshheading:9191839-Gene Expression, pubmed-meshheading:9191839-Genetic Vectors, pubmed-meshheading:9191839-Genome, Viral, pubmed-meshheading:9191839-Measles virus, pubmed-meshheading:9191839-Mice, pubmed-meshheading:9191839-Microscopy, Immunoelectron, pubmed-meshheading:9191839-Molecular Sequence Data, pubmed-meshheading:9191839-Nucleocapsid, pubmed-meshheading:9191839-Nucleoproteins, pubmed-meshheading:9191839-Phosphoproteins, pubmed-meshheading:9191839-RNA, Viral, pubmed-meshheading:9191839-Recombinant Fusion Proteins, pubmed-meshheading:9191839-Vaccinia virus, pubmed-meshheading:9191839-Vero Cells, pubmed-meshheading:9191839-Viral Proteins, pubmed-meshheading:9191839-Virion, pubmed-meshheading:9191839-Virus Assembly
pubmed:year
1997
pubmed:articleTitle
The assembly of the measles virus nucleoprotein into nucleocapsid-like particles is modulated by the phosphoprotein.
pubmed:affiliation
Etablissement de Transfusion Sanguine de Strasbourg, CIF INSERM 94-03, Stresbourg, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't