Linked Life Data

9190894

Source:http://linkedlifedata.com/resource/pubmed/id/9190894

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
1997-7-24
pubmed:abstractText
The Tax protein of the Human T-cell Leukemia Virus (HTLV) activates the expression of viral mRNA through a three 21 bp repeat enhancer located within the HTLV-1 LTR. Since Tax does not bind to the 21 bp DNA repeats directly, it has been speculated that Tax interacts with cellular protein(s) which mediate binding to the enhancer. We employed the yeast two hybrid system to identify host proteins that are potentially relevant to Tax transactivation. We identified a Tax binding protein encoded from a cDNA expression library derived from peripheral blood lymphocytes. The corresponding cDNA has sequence identity with a known transcription factor, activating factor-4 (ATF-4). ATF-4 also binds to GST-Tax fusion protein in vitro. Tax mutants that did not transactivate the HTLV-1 LTR also failed to bind ATF-4. The critical domain for Tax binding resides in a 85 amino acid stretch in the C-terminus of ATF-4, which contains the basic domain and leucine zipper. We further demonstrated that both full length and N-terminal truncated ATF-4 were able to enhance Tax transactivation. Thus, ATF-4 may act as an adapter between Tax and the TRE (Tax responsive element), and play an important role in Tax-mediated transactivation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2785-92
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:9190894-Activating Transcription Factor 4, pubmed-meshheading:9190894-Amino Acid Sequence, pubmed-meshheading:9190894-Animals, pubmed-meshheading:9190894-Cloning, Molecular, pubmed-meshheading:9190894-DNA, Complementary, pubmed-meshheading:9190894-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:9190894-Gene Products, tax, pubmed-meshheading:9190894-Genomic Library, pubmed-meshheading:9190894-HIV Long Terminal Repeat, pubmed-meshheading:9190894-Human T-lymphotropic virus 1, pubmed-meshheading:9190894-Humans, pubmed-meshheading:9190894-Mice, pubmed-meshheading:9190894-Molecular Sequence Data, pubmed-meshheading:9190894-Protein Binding, pubmed-meshheading:9190894-Transcription Factors, pubmed-meshheading:9190894-Transfection, pubmed-meshheading:9190894-Tumor Cells, Cultured
pubmed:year
1997
pubmed:articleTitle
Functional interaction of the HTLV-1 transactivator Tax with activating transcription factor-4 (ATF4).
pubmed:affiliation
Department of Medicine, University of California San Diego, La Jolla 92093-0665, USA.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.