Linked Life Data

9186916

Source:http://linkedlifedata.com/resource/pubmed/id/9186916

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1997-7-29
pubmed:abstractText
The purpose of this study was to evaluate the mechanisms of apolipoprotein B (apoB) modification during oxidation of human low density lipoproteins (LDL) mediated either by copper or by hypochlorite (HOCl). The kinetics of protein carbonyl formation, the relationship of apoB carbonyl formation to lipid peroxidation, and the loss of apoB lysine residues were determined. During copper-mediated LDL oxidation, apoB carbonyls appeared to increase slowly, displayed saturation kinetics in response to increasing copper concentrations, and correlated with lipid peroxidation. During HOCl-mediated LDL oxidation, apoB carbonyls increased with increasing HOCl concentrations reaching plateau with time; however, lipid peroxidation was not observed. During copper-mediated but not during HOCl-mediated LDL oxidation, LDL vitamin E was depleted. ApoB carbonyls formed more efficiently during copper-mediated LDL oxidation at low (< 5 microM) copper concentrations compared with higher copper concentrations or during HOCl-mediated LDL oxidation. The differences in oxidation kinetics between copper- and HOCl-mediated LDL oxidation support the concept that the binding of copper to LDL is a site specific process, and suggest that HOCl modifies apoB amino acids randomly.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-2275
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
992-1001
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Comparison between copper-mediated and hypochlorite-mediated modifications of human low density lipoproteins evaluated by protein carbonyl formation.
pubmed:affiliation
Department of Molecular and Cell Biology, University of California at Berkeley 94720-3200, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't