Linked Life Data

9183857

Source:http://linkedlifedata.com/resource/pubmed/id/9183857

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1997-8-7
pubmed:abstractText
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry relies on the formation of intact molecular ions to determine molecular weight. In biochemical research, conventional methods of protein analysis at picomol to fentomol sensitivity, such as sodium dodecyl sulphate polyacrylamide gel electrophoresis, have been replaced by this new ionization method. Unfortunately, problems caused by the mass accuracy and low resolution restrict the use of this ionization technique, particularly when a high mass accuracy in a high mass range is required. In this paper it is shown that the appropriate choice of parameters which determine the desorption/ionization of glycoproteins can improve the quality of MALDI mass spectra as well as mass reproducibility and resolution. The study of sample-matrix solution composition, pH and instrumental conditions allow the molecular weight determination of highly glycosylated proteins with a high percentage of sialic acid, e.g. erythropoietin. The glycosylation of this molecule which interferes with the production of multiply charged ions in electrospray ionization does not affect the desorption/ionization in MALDI analysis. We report the best operating conditions used to establish the degree of heterogeneity of erythropoietin.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0951-4198
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
907-13
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Matrix performance in matrix-assisted laser desorption/ionization for molecular weight determination in sialyl and non-sialyl oligosaccharide proteins.
pubmed:affiliation
Laboratorio di Igiene Ambientale e Tossicologia Industriale Fondazione S. Maugeri, Pavia, Italy.
pubmed:publicationType
Journal Article