Linked Life Data

9182606

Source:http://linkedlifedata.com/resource/pubmed/id/9182606

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-9-4
pubmed:abstractText
Transforming growth factor-betas (TGF-betas) have been associated with the development and maintenance of articular cartilage. However, no studies have addressed their role in the postnatal development of mandibular condylar cartilage. This investigation represents the first immunohistochemical characterization of TGF-beta isoforms and type II collagen in porcine mandibular condylar cartilage from various age groups. Furthermore, it is the first description of possible age-related changes in the expression of these proteins during postnatal development of this tissue. Condylar cartilage was dissected from freshly harvested temporomandibular joints of newborn, 6-, 12-, 24-, and 36-month-old farm swine. TGF-beta1, TGF-beta2, TGF-beta3, and type II collagen were localized via standard immunohistochemical procedures. An immunoblot technique was employed to compare the relative amount of each protein present in the various age groups. Immunoreactivity was detected in mandibular condylar cartilage for all three isoforms of TGF-beta and for Type II collagen. All age groups demonstrated some evidence of immunostaining, primarily in the cytoplasm of cells from most zones of the cartilage. Immunoblot results indicated that TGF-beta isoforms had individualized patterns of expression. When newborn protein levels were taken as the baseline, TGF-beta1 demonstrated a significant increase at ages 24 and 36 months. TGF-beta2 significantly increased at 6, 12, 24, and 36 months (peak levels at 24 months; similar levels at 6, 12, and 36 months), whereas TGF-beta3 remained stable at all ages. Type II collagen demonstrated increases that paralleled the increased levels of TGF-beta1 and TGF-beta2 at 24 and 36 months.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0302-766X
pubmed:author
pubmed:issnType
Print
pubmed:volume
289
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
119-24
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Type II collagen and TGF-betas in developing and aging porcine mandibular condylar cartilage: immunohistochemical studies.
pubmed:affiliation
Department of Orthodontics, Baylor College of Dentistry, P.O. Box 660677, Dallas, TX 75266-0677, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't