| pubmed-article:9174670 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9174670 | lifeskim:mentions | umls-concept:C0020731 | lld:lifeskim |
| pubmed-article:9174670 | lifeskim:mentions | umls-concept:C0018042 | lld:lifeskim |
| pubmed-article:9174670 | lifeskim:mentions | umls-concept:C0178539 | lld:lifeskim |
| pubmed-article:9174670 | lifeskim:mentions | umls-concept:C0014139 | lld:lifeskim |
| pubmed-article:9174670 | lifeskim:mentions | umls-concept:C0728899 | lld:lifeskim |
| pubmed-article:9174670 | lifeskim:mentions | umls-concept:C0598953 | lld:lifeskim |
| pubmed-article:9174670 | lifeskim:mentions | umls-concept:C0679209 | lld:lifeskim |
| pubmed-article:9174670 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:9174670 | pubmed:dateCreated | 1997-8-4 | lld:pubmed |
| pubmed-article:9174670 | pubmed:abstractText | The uptake mechanism of pertussis toxin (PT) in CHO and insulin-producing HIT-T15 cells was studied. By electron microscopy after direct labeling of the toxin with gold particles, PT was found to be taken up by receptor-mediated endocytosis. The presence of active pertussis toxin in the Golgi complex was shown by subcellular fractionation. The importance of the Golgi localization of pertussis toxin for the S1-dependent ADP-ribosylation of G-proteins was investigated employing Brefeldin A (BFA) treatment to disrupt Golgi structures. Treatment with Brefeldin A completely blocked the pertussis toxin mediated ADP-ribosylation of cellular G-proteins in CHO and HIT-T15 cells, whereas the BFA-resistant MDCK cells were not protected. A mutant CHO cell line (V24.1) exhibiting a temperature-sensitive Golgi complex could be protected when grown at restrictive conditions. These results strongly indicate that retrograde transport to the Golgi network is a necessary prerequisite for pertussis toxin mediated ADP-ribosylation of G-proteins and thus also for cellular intoxication. | lld:pubmed |
| pubmed-article:9174670 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9174670 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9174670 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9174670 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9174670 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9174670 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9174670 | pubmed:month | May | lld:pubmed |
| pubmed-article:9174670 | pubmed:issn | 0171-9335 | lld:pubmed |
| pubmed-article:9174670 | pubmed:author | pubmed-author:RobenekHH | lld:pubmed |
| pubmed-article:9174670 | pubmed:author | pubmed-author:SchmidtM AMA | lld:pubmed |
| pubmed-article:9174670 | pubmed:author | pubmed-author:LinnemannRR | lld:pubmed |
| pubmed-article:9174670 | pubmed:author | pubmed-author:el BayâAA | lld:pubmed |
| pubmed-article:9174670 | pubmed:author | pubmed-author:von... | lld:pubmed |
| pubmed-article:9174670 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9174670 | pubmed:volume | 73 | lld:pubmed |
| pubmed-article:9174670 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9174670 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9174670 | pubmed:pagination | 40-8 | lld:pubmed |
| pubmed-article:9174670 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:9174670 | pubmed:meshHeading | pubmed-meshheading:9174670-... | lld:pubmed |
| pubmed-article:9174670 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9174670 | pubmed:articleTitle | Endocytosis and retrograde transport of pertussis toxin to the Golgi complex as a prerequisite for cellular intoxication. | lld:pubmed |
| pubmed-article:9174670 | pubmed:affiliation | Institut für Infektiologie, Zentrum für Molekularbiologie der Entzündung (ZMBE) der Westfälischen Wilhelms-Universität, Münster/Germany. | lld:pubmed |
| pubmed-article:9174670 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9174670 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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