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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1997-6-30
|
| pubmed:abstractText |
The induction of peripheral tolerance following oral antigen administration in several autoimmune disease and conventional animal models correlates with the production of transforming growth factor-beta (TGF-beta) and T helper type 2 (Th2) cytokines. The factors regulating TGF-beta production and its relation to the Th2 response, however, have not been defined. We demonstrate that the systemic administration of antibodies to interleukin (IL)-12 to ovalbumin (OVA)-T cell receptor (TCR) transgenic mice fed high doses of OVA, followed by systemic OVA challenge, substantially enhances TGF-beta, but not IL-4 production by peripheral T cells. Furthermore, we demonstrate in an in vitro T cell differentiation model that naive (CD4+/Mel-14hi) OVA-TCR-T cells stimulated with OVA-pulsed dendritic cells (DC) produce four- to fivefold higher amounts of TGF-beta when cultured with anti-IL-12 or anti-interferon-gamma (IFN-gamma). In this in vitro system, IL-4 was not required for TGF-beta production by T cells; however, it appeared to enhance levels of TGF-beta by promoting the growth of TGF-beta-producing cells. Our findings demonstrate that IL-12 and IFN-gamma are important negative regulators of TGF-beta production both in vivo and in vitro, and that their modulation may be of benefit for the treatment of autoimmune disorders.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Blocking,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Ovalbumin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0014-2980
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
27
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1213-20
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9174613-Adjuvants, Immunologic,
pubmed-meshheading:9174613-Animals,
pubmed-meshheading:9174613-Antibodies, Blocking,
pubmed-meshheading:9174613-Antibodies, Monoclonal,
pubmed-meshheading:9174613-Cells, Cultured,
pubmed-meshheading:9174613-Coculture Techniques,
pubmed-meshheading:9174613-Dendritic Cells,
pubmed-meshheading:9174613-Female,
pubmed-meshheading:9174613-Interferon-gamma,
pubmed-meshheading:9174613-Interleukin-12,
pubmed-meshheading:9174613-Interleukin-4,
pubmed-meshheading:9174613-Mice,
pubmed-meshheading:9174613-Mice, Inbred BALB C,
pubmed-meshheading:9174613-Mice, Inbred C57BL,
pubmed-meshheading:9174613-Mice, Transgenic,
pubmed-meshheading:9174613-Ovalbumin,
pubmed-meshheading:9174613-Receptors, Antigen, T-Cell,
pubmed-meshheading:9174613-T-Lymphocytes,
pubmed-meshheading:9174613-Transforming Growth Factor beta
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Regulation of transforming growth factor-beta production by interleukin-12.
|
| pubmed:affiliation |
Mucosal Immunity Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-1890, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|