Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-6-3
pubmed:abstractText
Human renin has been expressed in Sf9 and CHO cells using two different gene constructs. The first construct contained a foreign signal peptide fused directly to the sequence encoding mature renin, whereas the second construct harbors the sequence for preprorenin. Prorenin was produced in significantly higher amounts than the mature enzyme expressed without its propeptide in both expression systems. Both directly expressed mature renin and proteolytically derived active renin have been purified and cocrystallized with the renin inhibitor Ro 42-5892. The 3D structure has been solved for both versions and demonstrates identity despite different glycosylation and different N termini.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1046-5928
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
81-91
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9172787-Amino Acid Sequence, pubmed-meshheading:9172787-Animals, pubmed-meshheading:9172787-Baculoviridae, pubmed-meshheading:9172787-Binding, Competitive, pubmed-meshheading:9172787-Blotting, Western, pubmed-meshheading:9172787-CHO Cells, pubmed-meshheading:9172787-Cell Line, pubmed-meshheading:9172787-Chromatography, Affinity, pubmed-meshheading:9172787-Cricetinae, pubmed-meshheading:9172787-Crystallization, pubmed-meshheading:9172787-Enzyme Precursors, pubmed-meshheading:9172787-Gene Expression, pubmed-meshheading:9172787-Humans, pubmed-meshheading:9172787-Imidazoles, pubmed-meshheading:9172787-Mass Spectrometry, pubmed-meshheading:9172787-Models, Molecular, pubmed-meshheading:9172787-Molecular Sequence Data, pubmed-meshheading:9172787-Peptide Fragments, pubmed-meshheading:9172787-Protein Conformation, pubmed-meshheading:9172787-Radioimmunoassay, pubmed-meshheading:9172787-Recombinant Proteins, pubmed-meshheading:9172787-Renin, pubmed-meshheading:9172787-Spodoptera
pubmed:year
1996
pubmed:articleTitle
Recombinant human renin produced in different expression systems: biochemical properties and 3D structure.
pubmed:affiliation
Pharma Division, Preclinical Research, F. Hoffmann-La Roche, Ltd., Basel, Switzerland.
pubmed:publicationType
Journal Article