Linked Life Data

9171217

Source:http://linkedlifedata.com/resource/pubmed/id/9171217

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1997-6-20
pubmed:abstractText
Polymerase chain reaction-based limiting dilution assays (PLDAs), commonly called end-point dilutions, are frequently used to quantify the copy numbers of human immunodeficiency virus (HIV) and other viruses in biological samples; however, the way in which these assays are done, and the mathematical method used to estimate copy numbers, vary from laboratory to laboratory. Here, we describe a statistical method for estimating the number of copies and the associated standard error of the estimate, using a PLDA. The copy number is estimated by the value that maximizes the goodness of fit between the observed numbers of negative reactions and the expected numbers of negative reactions (the latter estimated using a Poisson probability distribution) as measured by the chi2 statistic. The method described here also takes into account user-specified probabilities of obtaining a false-positive or a false-negative PCR result, a feature that is not generally available with other limiting dilution estimation procedures. QUALITY, a computer program that implements the estimation strategy, is also described. Simulations illustrate the efficiency of estimation with different numbers of PCR amplifications conducted at each dilution, and different dilution factors. Finally, a simple strategy for more efficient assays is proposed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0889-2229
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
737-42
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Quantitation of target molecules from polymerase chain reaction-based limiting dilution assays.
pubmed:affiliation
Department of Microbiology, University of Washington School of Medicine, Seattle 98195-7740, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't