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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1997-6-24
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pubmed:abstractText |
MDCK cells were stably transfected with rat G alpha o cDNA and confluent polarized monolayers were analyzed by immunocytochemistry to compare the intracellular targeting of G alpha o with the localization of the endogenously expressed G alpha i subunits. Immunofluorescence confocal microscopy showed that G alpha o is targeted strictly to the lateral membrane. Immunolocalization of G alpha i-1, -2, and -3 showed that G alpha i-1 and -2 are confined to the cytoplasm and G alpha i-3 is found on the lateral membrane, in the cytoplasm, and faintly on the apical surface of these cells. Thus, the different pertussis toxin-sensitive G-proteins are differentially localized in polarized epithelial cells.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
234
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1-7
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9168949-Animals,
pubmed-meshheading:9168949-Antibodies, Monoclonal,
pubmed-meshheading:9168949-Cell Line,
pubmed-meshheading:9168949-Cell Membrane,
pubmed-meshheading:9168949-Cell Polarity,
pubmed-meshheading:9168949-Cytoplasm,
pubmed-meshheading:9168949-DNA, Complementary,
pubmed-meshheading:9168949-Dogs,
pubmed-meshheading:9168949-Epithelium,
pubmed-meshheading:9168949-Fluorescent Antibody Technique,
pubmed-meshheading:9168949-GTP-Binding Proteins,
pubmed-meshheading:9168949-Gene Expression,
pubmed-meshheading:9168949-Gene Targeting,
pubmed-meshheading:9168949-Immunoblotting,
pubmed-meshheading:9168949-Kidney,
pubmed-meshheading:9168949-Microscopy, Confocal,
pubmed-meshheading:9168949-Pertussis Toxin,
pubmed-meshheading:9168949-Rats,
pubmed-meshheading:9168949-Transfection,
pubmed-meshheading:9168949-Virulence Factors, Bordetella
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pubmed:year |
1997
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pubmed:articleTitle |
Differential localization of G-proteins, G alpha o and G alpha i-1, -2, and -3, in polarized epithelial MDCK cells.
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pubmed:affiliation |
Department of Pharmacology, University of Washington School of Medicine, Seattle 98195, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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