Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1997-6-17
|
| pubmed:abstractText |
Signaling via intercellular junctions plays an important role in the regulation of growth and differentiation of epithelial cells. Loss of cell-cell contacts has been implicated in carcinogenesis, tumor progression, and metastasis. Here, we investigated whether 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] was able to stimulate the assembly of adherens junctions and/or desmosomes in cultured human keratinocytes. After 4-day incubation, 1,25-(OH)2D3 caused assembly of adherens junctions, but not desmosomes. The adherens junctions were identified upon known ultrastructural criteria and evidence of the translocation of specific junctional proteins (E-cadherin, P-cadherin, alpha-catenin, and vinculin) to the cell-cell borders. The presence of alpha-catenin and vinculin at cell-cell borders indicated that the adherens junctions were functional. This was further supported by showing that anti E-cadherin antibody inhibited the 1,25-(OH)2D3-induced keratinocyte stratification. A relation between protein kinase C and adherens junction regulation was noticed. 1,25-(OH)2D3-dependent formation of junctions was blocked by the inhibitors of protein kinase C, bisindolylmaleimide and 1-(5-isoquinolinylsulfonyl)-2-methyl-piperazine (H-7), and treatment of keratinocytes with 1,25-(OH)2D3 caused a rapid activation of protein kinase C and its translocation to the membranes. Formation of intercellular contacts may be an important mechanism of 1,25-(OH)2D3 action in hyperproliferative and neoplastic diseases.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0013-7227
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
138
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2241-8
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9165007-1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine,
pubmed-meshheading:9165007-Analysis of Variance,
pubmed-meshheading:9165007-Cadherins,
pubmed-meshheading:9165007-Calcitriol,
pubmed-meshheading:9165007-Cell Adhesion,
pubmed-meshheading:9165007-Cell Membrane,
pubmed-meshheading:9165007-Cells, Cultured,
pubmed-meshheading:9165007-Cryopreservation,
pubmed-meshheading:9165007-Cytosol,
pubmed-meshheading:9165007-Desmosomes,
pubmed-meshheading:9165007-Humans,
pubmed-meshheading:9165007-Infant, Newborn,
pubmed-meshheading:9165007-Intercellular Junctions,
pubmed-meshheading:9165007-Keratinocytes,
pubmed-meshheading:9165007-Microscopy, Electron,
pubmed-meshheading:9165007-Protein Kinase C
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
1,25-dihydroxyvitamin D3 stimulates the assembly of adherens junctions in keratinocytes: involvement of protein kinase C.
|
| pubmed:affiliation |
Department of Dermatological Research, Leo Pharmaceutical Products, Ballerup, Denmark.
|
| pubmed:publicationType |
Journal Article
|