Linked Life Data

9155681

Source:http://linkedlifedata.com/resource/pubmed/id/9155681

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1997-5-29
pubmed:abstractText
The control of methicillin resistant Staphylococcus aureus (MRSA) relies on the rapid and sensitive detection of carriage. The roles of an enrichment broth, duration of incubation, and Baird-Parker medium containing ciprofloxacin (BPC) were evaluated in comparison with standard media in a centre where the prevalence of ciprofloxacin resistance among MRSA is over 98%. Screening swabs from 402 sites were plated onto BPC, mannitol salt agar (MSA), and MSA with methicillin (MMSA). The swabs were enriched in Tryptone-T broth with 6% salt for 24 hours and the broths subcultured onto BPC, MSA, and MMSA. MRSA was isolated from 134 swabs. Significantly more isolates were obtained by incubating culture plates for 42 hours rather than 18 hours, by the use of broth enrichment, and by addition of methicillin or ciprofloxacin to media. BPC was the most sensitive medium (107 isolates (80%) by direct culture at 42 hours), grew the fewest contaminants, and allowed provisional reporting of 73% of isolates at 18 hours by colonial appearance and use of Staphaurex Plus rapid latex reagent. This may allow the introduction of infection control measures a day earlier than when other established methods are used.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9746
pubmed:author
pubmed:issnType
Print
pubmed:volume
50
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
257-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Comparison of methods for the isolation of methicillin resistant Staphylococcus aureus.
pubmed:affiliation
Public Health Laboratory, Sheffield.
pubmed:publicationType
Journal Article, Comparative Study