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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1997-7-18
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pubmed:abstractText |
1. The generation of superoxide anions (O2-) by intact pig coronary artery rings was measured using a lucigenin-enhanced chemiluminescence technique and a histochemical technique with Nitroblue Tetrazolium (NBT) staining. 2. Isolated arteries with intact endothelium generated O2- at a rate of 9.0 +/- 0.8 pmol min-1 (mg dry weight)-1; this rate was diminished by about 24% when the endothelium was removed. The NBT staining of arterial ring preparations showed formazan precipitation mainly in the intima. Arterial rings were pretreated with diethylthiocarbamate in order to inhibit Cu-Zn superoxide dismutase (SOD) activity which increased the O2- generation by 184 +/- 55% (n = 10; P < 0.01). Stimulation of protein kinase C with phorbol 12-myristate 13-acetate (5 microM) enhanced endothelium-dependent O2- generation by 136 +/- 20% (n = 19; P < 0.01). Neither stimulation with bradykinin or substance P, nor inhibition with NG-nitro-L-arginine methyl ester of endothelial nitric oxide synthase had a significant effect on O2- generation. In contrast, the inhibition of flavoproteins with diphenyliodonium decreased concentration-dependent O2- generation (IC50, 1.85 +/- 5.33 microM). Inhibition of tetrahydrobiopterin synthesis with 2,4-diamino-6-hydroxy-pyrimidine resulted in a reduced generation of O2- by about 55%. 3. The addition of 100 microM NADH and 100 microM NADPH resulted in an excessive generation of O2- at a rate of 0.68 +/- 0.03 and 0.26 +/- 0.01 nmol O2- min-1 (mg protein)-1, respectively, in the membrane fraction, but not in the cytosolic fraction, of homogenates obtained from arteries. 4. The results suggest that intact coronary arteries do generate O2- under basal conditions and that the endothelial layer significantly contributes to this phenomenon. This generation of O2- is greatly influenced by intrinsic SOD activity. It is suggested that basal vascular O2- generation is mainly due to membrane-bound NAD(P)H oxidase activity and/or tetrahydrobiopterin-dependent processes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-1280257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-1654842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-1658794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-1707173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-1847199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-1847583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-1928390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-2156873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-2823632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-2830901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-3009494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-3021851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-3129432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-4377100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-4972775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-6095281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-6265407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-7504210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-7511072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-7513382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-7528647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-7529763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-7543827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-7578211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-7685338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-7686905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-7690193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-7898085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-8024019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-8103664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-8302829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-8390482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-8396109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-8396550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9147321-8439298
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
500 ( Pt 2)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
331-42
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:9147321-Acridines,
pubmed-meshheading:9147321-Animals,
pubmed-meshheading:9147321-Anions,
pubmed-meshheading:9147321-Arteries,
pubmed-meshheading:9147321-Cell-Free System,
pubmed-meshheading:9147321-Coronary Vessels,
pubmed-meshheading:9147321-Endothelium, Vascular,
pubmed-meshheading:9147321-Histocytochemistry,
pubmed-meshheading:9147321-Luminescent Measurements,
pubmed-meshheading:9147321-Nitroblue Tetrazolium,
pubmed-meshheading:9147321-Oxygen,
pubmed-meshheading:9147321-Superoxide Dismutase,
pubmed-meshheading:9147321-Superoxides,
pubmed-meshheading:9147321-Swine
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pubmed:year |
1997
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pubmed:articleTitle |
Endothelial-derived superoxide anions in pig coronary arteries: evidence from lucigenin chemiluminescence and histochemical techniques.
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pubmed:affiliation |
Division of Cardiology, Hannover Medical School, Germany.
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pubmed:publicationType |
Journal Article
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