Linked Life Data

9133440

Source:http://linkedlifedata.com/resource/pubmed/id/9133440

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-6-2
pubmed:abstractText
The outgrowth of the mesoderm of the developing limb bud in response to the apical ectodermal ridge (AER) is mediated at least in part by members of the FGF family. Recent studies have indicated that FGFs need to interact with heparan sulfate proteoglycans in order to bind to and activate their specific cell surface receptors. Syndecan-3 is an integral membrane heparan sulfate proteoglycan that is highly expressed by the distal mesodermal cells of the chick limb bud that are undergoing proliferation and outgrowth in response to the AER. Here we report that maintenance of high-level syndecan-3 expression by the subridge mesoderm of the chick limb bud is directly or indirectly dependent on the AER, since its expression is severely impaired in the distal mesoderm of the limb buds of limbless and wingless mutant embryos which lack functional AERs capable of directing the outgrowth of limb mesoderm. We have also found that exogenous FGF-2 maintains a domain of high-level syndecan-3 expression in the outgrowing mesodermal cells of explants of the posterior mesoderm of normal limb buds cultured in the absence of the AER and in the outgrowing subapical mesoderm of explants of limbless mutant limb buds which lack a functional AER. These results suggest that the domain of high-level syndecan-3 expression in the subridge mesoderm of normal limb buds is maintained by FGFs produced by the AER. Finally, we report that polyclonal antibodies against a syndecan-3 fusion protein inhibit the ability of FGF-2 to promote the proliferation and outgrowth of the posterior subridge mesoderm of limb buds cultured in the absence of the AER. These results suggest that syndecan-3 plays an essential role in limb outgrowth by mediating the interaction of FGFs produced by the AER with the underlying mesoderm of the limb bud.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0012-1606
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
184
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
343-50
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:9133440-Animals, pubmed-meshheading:9133440-Antibodies, pubmed-meshheading:9133440-Cell Division, pubmed-meshheading:9133440-Chick Embryo, pubmed-meshheading:9133440-DNA Probes, pubmed-meshheading:9133440-Ectoderm, pubmed-meshheading:9133440-Fibroblast Growth Factor 2, pubmed-meshheading:9133440-Gene Expression Regulation, Developmental, pubmed-meshheading:9133440-Immunohistochemistry, pubmed-meshheading:9133440-In Situ Hybridization, pubmed-meshheading:9133440-Limb Buds, pubmed-meshheading:9133440-Membrane Glycoproteins, pubmed-meshheading:9133440-Mesoderm, pubmed-meshheading:9133440-Mutation, pubmed-meshheading:9133440-Organ Culture Techniques, pubmed-meshheading:9133440-Proteoglycans, pubmed-meshheading:9133440-RNA, Messenger, pubmed-meshheading:9133440-Receptors, Fibroblast Growth Factor, pubmed-meshheading:9133440-Syndecan-3, pubmed-meshheading:9133440-Transcription, Genetic
pubmed:year
1997
pubmed:articleTitle
FGF-stimulated outgrowth and proliferation of limb mesoderm is dependent on syndecan-3.
pubmed:affiliation
Department of Anatomy, University of Connecticut Health Center, Farmington 06030, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.