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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1997-6-3
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pubmed:abstractText |
P-selectin (CD62P) is a Ca2+-dependent endogenous lectin that can be expressed by vascular endothelium and platelets. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1 (PSGL-1). P-selectin can also bind to carcinoma cells, but the nature of the ligand(s) on these cells is unknown. Here we investigated the P-selectin binding to a breast and a small cell lung carcinoma cell line that are negative for PSGL-1. We report that CD24, a mucin-type glycosylphosphatidylinositol-linked cell surface molecule on human neutrophils, pre B lymphocytes, and many tumors can promote binding to P-selectin. Latex beads coated with purified CD24 from the two carcinoma cell lines but also neutrophils could bind specifically to P-selectin-IgG. The binding was dependent on divalent cations and was abolished by treatment with O-sialoglycoprotein endopeptidase but not endoglycosidase F or sialidase. The beads were stained with a monoclonal antibody (MoAb) to CD57 (HNK-1 carbohydrate epitope) but did not react with MoAbs against the sialylLe(x/a) epitope. The carcinoma cells and CD24-beads derived from these cells could bind to activated platelets or P-selectin transfected Chinese hamster ovary cells (P-CHO) in a P-selectin-dependent manner and this binding was blocked by soluble CD24. Transfection of human adenocarcinoma cells with CD24 enhanced the P-selectin-dependent binding to activated platelets. Treatment of the carcinoma cells or the CD24 transfectant with phosphatidylinositol-specific phospholipase C reduced CD24 expression and P-selectin-IgG binding concomitantly. These results establish a role of CD24 as a novel ligand for P-selectin on tumor cells. The CD24/P-selectin binding pathway could be important in the dissimination of tumor cells by facilitating the interaction with platelets or endothelial cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD24,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD57,
http://linkedlifedata.com/resource/pubmed/chemical/CD24 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mucins,
http://linkedlifedata.com/resource/pubmed/chemical/P-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/P-selectin ligand protein
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-4971
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pubmed:author |
pubmed-author:AignerSS,
pubmed-author:AltevogtPP,
pubmed-author:FogelMM,
pubmed-author:RuppertMM,
pubmed-author:SammarMM,
pubmed-author:StahelRR,
pubmed-author:SthoegerZ MZM,
pubmed-author:VestweberDD,
pubmed-author:WeberEE,
pubmed-author:ZarnJJ,
pubmed-author:ZellerYY
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3385-95
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9129046-Amino Acid Sequence,
pubmed-meshheading:9129046-Animals,
pubmed-meshheading:9129046-Antibodies, Monoclonal,
pubmed-meshheading:9129046-Antigens, CD,
pubmed-meshheading:9129046-Antigens, CD24,
pubmed-meshheading:9129046-Antigens, CD57,
pubmed-meshheading:9129046-Base Sequence,
pubmed-meshheading:9129046-Binding Sites,
pubmed-meshheading:9129046-Blood Platelets,
pubmed-meshheading:9129046-Breast Neoplasms,
pubmed-meshheading:9129046-CHO Cells,
pubmed-meshheading:9129046-Cell Adhesion,
pubmed-meshheading:9129046-Chromatography, Affinity,
pubmed-meshheading:9129046-Cricetinae,
pubmed-meshheading:9129046-DNA Primers,
pubmed-meshheading:9129046-Epitopes,
pubmed-meshheading:9129046-Female,
pubmed-meshheading:9129046-Humans,
pubmed-meshheading:9129046-Immunoglobulin G,
pubmed-meshheading:9129046-Ligands,
pubmed-meshheading:9129046-Lung Neoplasms,
pubmed-meshheading:9129046-Membrane Glycoproteins,
pubmed-meshheading:9129046-Molecular Sequence Data,
pubmed-meshheading:9129046-Mucins,
pubmed-meshheading:9129046-Neutrophils,
pubmed-meshheading:9129046-P-Selectin,
pubmed-meshheading:9129046-Platelet Activation,
pubmed-meshheading:9129046-Polymerase Chain Reaction,
pubmed-meshheading:9129046-Sequence Homology, Amino Acid,
pubmed-meshheading:9129046-Tumor Cells, Cultured
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pubmed:year |
1997
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pubmed:articleTitle |
CD24, a mucin-type glycoprotein, is a ligand for P-selectin on human tumor cells.
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pubmed:affiliation |
Tumor Immunology Programme, German Cancer Research Center, Heidelberg.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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