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rdf:type |
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|
lifeskim:mentions |
umls-concept:C0002520,
umls-concept:C0019751,
umls-concept:C0021469,
umls-concept:C0022686,
umls-concept:C0022688,
umls-concept:C0085732,
umls-concept:C0205171,
umls-concept:C0441833,
umls-concept:C0597357,
umls-concept:C1415562,
umls-concept:C1416645,
umls-concept:C2587213
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|
pubmed:issue |
9
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|
pubmed:dateCreated |
1997-5-19
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pubmed:abstractText |
To examine the structural basis for the specific recognition of the MHC class I allotypes HLA-Cw*0401 and HLA-Cw*0304 by the killer cell inhibitory receptors (KIR) cl42 and cl43, respectively, mutant KIR-Ig fusion proteins were tested by direct binding to cells transfected with single HLA-C alleles. The putative loop region at position 44-46 of KIR contained amino acids that were necessary for the discrimination between HLA-Cw*0401 and HLA-Cw*0304. Surprisingly, exchanging the methionine at position 44 in cl42 with the lysine at position 44 in cl43 was sufficient to switch the specificity of cl42 from HLA-Cw*0401 to HLA-Cw*0304, and vice versa. Thus, a single amino acid in the first Ig domain of these KIR determines their ability to discriminate between the two groups of HLA-C allotypes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-1767
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
158
|
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4026-8
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:9126959-Alleles,
pubmed-meshheading:9126959-Amino Acid Sequence,
pubmed-meshheading:9126959-Consensus Sequence,
pubmed-meshheading:9126959-HLA-C Antigens,
pubmed-meshheading:9126959-Humans,
pubmed-meshheading:9126959-Killer Cells, Natural,
pubmed-meshheading:9126959-Molecular Sequence Data,
pubmed-meshheading:9126959-Mutagenesis, Site-Directed,
pubmed-meshheading:9126959-Receptors, Immunologic,
pubmed-meshheading:9126959-Receptors, KIR,
pubmed-meshheading:9126959-Receptors, KIR2DL3,
pubmed-meshheading:9126959-Recombinant Fusion Proteins,
pubmed-meshheading:9126959-Structure-Activity Relationship
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pubmed:year |
1997
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|
pubmed:articleTitle |
A single amino acid in the p58 killer cell inhibitory receptor controls the ability of natural killer cells to discriminate between the two groups of HLA-C allotypes.
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pubmed:affiliation |
Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA.
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pubmed:publicationType |
Journal Article
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