Linked Life Data

9124702

Source:http://linkedlifedata.com/resource/pubmed/id/9124702

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-4-23
pubmed:databankReference
pubmed:abstractText
The bovine leukaemia inhibitory factor was isolated from a phage library and sequences for the gene, in addition to 1213 bp of 5' and 432 bp of 3' sequences, were obtained and compared with other mammalian leukaemia inhibitory factor genes. Comparisons indicated amino acid homologies ranging from 89.6% to 77.2% with the human and mouse homologues, respectively. Analysis of 500 bp of 5' regulatory regions indicated homologies ranging from 83.6% to 74.4% with the corresponding human and sheep sequences, respectively. Additionally, bovine leukaemia inhibitory factor-specific primers were prepared, and a panel of bovine x hamster somatic cell lines were analysed by the polymerase chain reaction (PCR). Data indicated 93% concordance of leukaemia inhibitory factor with aldehyde dehydrogenase 2 located on bovine chromosome 17, and concordance of 81% with myelin basic protein situated on bovine chromosome 24. Southern analysis of selected hybrids confirmed the PCR results, thus conclusively assigning the bovine leukaemia inhibitory factor gene to chromosome 17. Sequence analysis also revealed a microsatellite in intron 2 of the bovine leukaemia inhibitory factor. Analysis of this region by PCR in 22 unrelated Bos taurus and 19 unrelated Bos indicus cattle detected nine different alleles. Polymorphic information content values were 0.53 and 0.80 in B. taurus and B. indicus, respectively. Additionally, the same leukaemia inhibitory factor primers successfully detected allelic variants at this locus in Bos javanicus, Bos guarus and Bison bison but not in Odocoileus virginianus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0268-9146
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
14-20
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9124702-Amino Acid Sequence, pubmed-meshheading:9124702-Animals, pubmed-meshheading:9124702-Base Sequence, pubmed-meshheading:9124702-Cattle, pubmed-meshheading:9124702-Chromosome Mapping, pubmed-meshheading:9124702-Cloning, Molecular, pubmed-meshheading:9124702-Genes, pubmed-meshheading:9124702-Growth Inhibitors, pubmed-meshheading:9124702-Interleukin-6, pubmed-meshheading:9124702-Introns, pubmed-meshheading:9124702-Leukemia Inhibitory Factor, pubmed-meshheading:9124702-Lymphokines, pubmed-meshheading:9124702-Microsatellite Repeats, pubmed-meshheading:9124702-Molecular Sequence Data, pubmed-meshheading:9124702-Polymorphism, Restriction Fragment Length, pubmed-meshheading:9124702-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:9124702-Sequence Analysis, DNA, pubmed-meshheading:9124702-Sequence Homology, Amino Acid
pubmed:year
1997
pubmed:articleTitle
Genetic characterization of the bovine leukaemia inhibitory factor (LIF) gene: isolation and sequencing, chromosome assignment and microsatellite analysis.
pubmed:affiliation
Department of Veterinary Anatomy and Public Health, College of Veterinary Medicine, Texas A&M University, College Station 77843-4458, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't