Linked Life Data

9124336

Source:http://linkedlifedata.com/resource/pubmed/id/9124336

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2 Pt 1
pubmed:dateCreated
1997-4-24
pubmed:abstractText
It is clear that alpha-melanocyte-stimulating hormone (alpha-MSH), released by the hypophysial neurointermediate lobe, is a mediator of suckling-induced prolactin release, but several questions surrounding its role remain unresolved. Accordingly, the objectives of the present study were 1) to establish whether alpha-MSH could bind in a reversible manner to a specific secretory type cell within the adenohypophysis (AP), 2) to resolve the issue of whether the peptide could compete with dopamine for the same receptor binding site, and 3) to seek a functional signaling correlate for alpha-MSH binding. In pursuit of these objectives, we subjected pituitary cells from lactating rats to alpha-MSH receptor autoradiography, AP hormone immunocytochemistry, or digital imaging fluorescence microscopy with fura 2 as a calcium-sensitive probe. Our results show that alpha-MSH binding is restricted to mammotropes and that a specific subpopulation of these express functional alpha-MSH receptors that are coupled to a Ca2+ signaling pathway. Moreover, alpha-MSH does not compete with dopamine antagonists/agonists for the same binding site.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
272
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
E282-7
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Phenotypic characterization and functional correlation of alpha-MSH binding to pituitary cells.
pubmed:affiliation
Department of Cell Biology and Anatomy, Medical University of South Carolina, Charleston 29425, USA.
pubmed:publicationType
Journal Article