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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1997-4-24
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pubmed:abstractText |
Significant cleavage by hammerhead ribozymes requires activation by divalent metal ions. Several models have been proposed to account for the influence of metal ions on hammerhead activity. A number of recent papers have presented data that have been interpreted as supporting a one-metal-hydroxide-ion mechanism. In addition, a solvent deuterium isotope effect has been taken as evidence against a proton transfer in the rate-limiting step of the cleavage reaction. We propose that these data are more easily explained by a two-metal-ion mechanism that does not involve a metal hydroxide, but does involve a proton transfer in the rate-limiting step.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9122187-1709484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9122187-1716459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9122187-1989886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9122187-2036355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9122187-2036380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9122187-2235484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9122187-3194400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9122187-3594567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9122187-3907691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9122187-7479038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9122187-7541315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9122187-7783134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9122187-7969422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9122187-8136375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9122187-8241158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9122187-8341661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9122187-8611518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9122187-8744356
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
94
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2290-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9122187-Base Sequence,
pubmed-meshheading:9122187-Binding Sites,
pubmed-meshheading:9122187-Calcium,
pubmed-meshheading:9122187-Catalysis,
pubmed-meshheading:9122187-Cations, Divalent,
pubmed-meshheading:9122187-Cobalt,
pubmed-meshheading:9122187-Crystallography, X-Ray,
pubmed-meshheading:9122187-Kinetics,
pubmed-meshheading:9122187-Lead,
pubmed-meshheading:9122187-Magnesium,
pubmed-meshheading:9122187-Manganese,
pubmed-meshheading:9122187-Molecular Sequence Data,
pubmed-meshheading:9122187-Nucleic Acid Conformation,
pubmed-meshheading:9122187-RNA, Catalytic,
pubmed-meshheading:9122187-Thionucleotides
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pubmed:year |
1997
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pubmed:articleTitle |
Observations on catalysis by hammerhead ribozymes are consistent with a two-divalent-metal-ion mechanism.
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pubmed:affiliation |
Department of Chemistry, University of Oregon, Eugene 97403-1129, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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