Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1977-12-29
pubmed:abstractText
Previous calculations using crystal structure coordinates (Strickland and Mercola [1976], Biochemistry. 15: 3857) have predicted that about 40 percent of the calculated tyrosyl circular dichroism of hexameric insulin is due to one of the four tyrosine residues: viz. the A14-tyrosine interacting with the nearby B1-phenylalanine ring group. We have tested this prediction by measuring the tyrosyl circular dichroism of an isomorphous analogue of insulin, des-B1-phenylalanine-insulin. Contrary to expectation, the resulting circular dichroism was the same as that of insulin. It is concluded that the B1-phenylalanine residue does not in fact make a large contribution to the circular dichroism of A14-tyrosine. This result is probably due to the thermal motion of the B1 and A14 ring groups not taken into account by the calculations. An example of the effects of thermal motion on the calculated circular dichroism is given and improvements that do take into account thermal motion are discussed.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/911983-1175610, http://linkedlifedata.com/resource/pubmed/commentcorrection/911983-1176725, http://linkedlifedata.com/resource/pubmed/commentcorrection/911983-1272390, http://linkedlifedata.com/resource/pubmed/commentcorrection/911983-1278422, http://linkedlifedata.com/resource/pubmed/commentcorrection/911983-235463, http://linkedlifedata.com/resource/pubmed/commentcorrection/911983-4365215, http://linkedlifedata.com/resource/pubmed/commentcorrection/911983-4473202, http://linkedlifedata.com/resource/pubmed/commentcorrection/911983-4508321, http://linkedlifedata.com/resource/pubmed/commentcorrection/911983-4591332, http://linkedlifedata.com/resource/pubmed/commentcorrection/911983-4673054, http://linkedlifedata.com/resource/pubmed/commentcorrection/911983-4735299, http://linkedlifedata.com/resource/pubmed/commentcorrection/911983-4932997, http://linkedlifedata.com/resource/pubmed/commentcorrection/911983-5066442, http://linkedlifedata.com/resource/pubmed/commentcorrection/911983-5101635, http://linkedlifedata.com/resource/pubmed/commentcorrection/911983-5460180, http://linkedlifedata.com/resource/pubmed/commentcorrection/911983-5560471, http://linkedlifedata.com/resource/pubmed/commentcorrection/911983-5658118, http://linkedlifedata.com/resource/pubmed/commentcorrection/911983-5726558, http://linkedlifedata.com/resource/pubmed/commentcorrection/911983-5799711, http://linkedlifedata.com/resource/pubmed/commentcorrection/911983-986169
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
233-43
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
Side-chain mobility and the calculation of tyrosyl circular dichroism of proteins. Implications of a test with insulin and des-B1-phenylalanine insulin.
pubmed:publicationType
Journal Article