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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1997-5-22
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pubmed:abstractText |
The interleukin-2 (IL-2) receptor (IL-2R) is composed of three subunits. Of these, IL-2Ra is required for high-affinity IL-2 binding, while IL-2R beta and IL-2R gamma(c) are required for the transduction of IL-2-generated signals. Signals transduced via the S region of the IL-2R beta (amino acids 267-322) in BAF/3 cells activate the phosphatidylinositol 3-kinase (PI3-kinase) and induce the expression of Bcl-2 and c-myc. Through the induction of Bcl-2, IL-2 inhibits apoptosis and through the combination of Bcl-2 and c-myc it stimulates progression through the cell cycle. Here we show that the protein kinase encoded by the Akt proto-oncogene is activated by IL-2. Akt activation by IL-2 depends on PI3-kinase signals transduced via the S region of the IL-2R beta and is linked to the translocation of Akt to the cell membrane. Expression of catalytically active Akt mutants in BAF/3 cells expressing IL-2R beta[A0]delta S promotes the expression of Bcl-2 and c-myc, inhibits apoptosis induced by IL-3 deprivation or staurosporine, and stimulates cell cycle progression. The same mutants also stimulate cell cycle progression in 2780a, an IL-2-dependent T cell line that undergoes G1 arrest rather than apoptosis after IL-2 deprivation. The activation of Akt by IL-2 via the PI3-kinase and the rescue of the PI3-kinase-mediated antiapoptotic and proliferative IL-2 signals by catalytically active Akt indicate that these signals are transduced by Akt.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-1460037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-2196385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-3920530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-7484461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-7486666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-7600304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-7612228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-7637799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-7637810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-7736574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-7774014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-7891724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-7973659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-8025955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-8026467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-8134114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-8294403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-8313896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-8399352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-8440263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-8510938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-8524310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-8524413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-8617712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-8667646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-8754810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-8791418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-8887656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-8940066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-8962093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9108028-8972214
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
94
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3627-32
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9108028-Animals,
pubmed-meshheading:9108028-Apoptosis,
pubmed-meshheading:9108028-Cell Division,
pubmed-meshheading:9108028-Cell Line,
pubmed-meshheading:9108028-Interleukin-2,
pubmed-meshheading:9108028-Protein-Serine-Threonine Kinases,
pubmed-meshheading:9108028-Proto-Oncogene Proteins,
pubmed-meshheading:9108028-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:9108028-Rats,
pubmed-meshheading:9108028-Signal Transduction,
pubmed-meshheading:9108028-T-Lymphocytes
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pubmed:year |
1997
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pubmed:articleTitle |
Transduction of interleukin-2 antiapoptotic and proliferative signals via Akt protein kinase.
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pubmed:affiliation |
Fox Chase Cancer Center, Philadelphia, PA 19111, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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