Linked Life Data

9101410

Source:http://linkedlifedata.com/resource/pubmed/id/9101410

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-6-10
pubmed:abstractText
The recent identification of genes encoding melanoma-associated antigens has opened new possibilities for the development of cancer vaccines designed to cause the rejection of established tumors. To develop a syngeneic animal model for evaluating antigen-specific vaccines in cancer therapy, the murine homologues of the human melanoma antigens MART1 and gp100, which were specifically recognized by tumor-infiltrating lymphocytes from patients with melanoma, were cloned and sequenced from a murine B16 melanoma cDNA library. The open reading frames of murine MART1 and gp100 encode proteins of 113- and 626-amino acids with 68.8 and 77% identity to the respective human proteins. Comparison of the DNA sequences of the murine MART1 genes, derived from normal melanocytes, the immortalized nontumorgenic melanocyte line Melan-a and the B16 melanoma, showed all to be identical. Northern and Western blot analyses confirmed that both genes encoded products that were melanocyte lineage proteins. Mice immunized with murine MART1 or gp100 using recombinant vaccinia virus failed to produce any detectable T-cell responses or protective immunity against B16 melanoma. In contrast, immunization of mice with human gp100 using recombinant adenoviruses elicited T cells specific for hgp100, but these T cells also cross reacted with B16 tumor in vitro and induced significant but weak protection against B16 challenge. Immunization with human and mouse gp100 together [adenovirus type 2 (Ad2)-hgp100 plus recombinant vaccinia virus (rVV)-mgp100], or immunization with human gp100 (Ad2-hgp100) and boosting with heterologous vector (rVV-hgp100 or rVV-mgp100) or homologous vector (Ad2-hgp100), did not significantly enhance the protective response against B16 melanoma. These results may suggest that immunization with heterologous tumor antigen, rather than self, may be more effective as an immunotherapeutic reagent in designing antigen-specific cancer vaccines.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-1321851, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-7516411, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-7534800, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-7536009, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-7590818, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-7606778, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-7706734, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-7718089, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-7722321, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-7751637, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-7761347, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-7836932, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-7870580, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-7888073, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-7937789, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-7961886, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-8006576, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-8006593, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-8018207, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-8022805, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-8170938, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-8301145, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-8340755, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-8381158, http://linkedlifedata.com/resource/pubmed/commentcorrection/9101410-8543823
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1524-9557
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15-25
pubmed:dateRevised
2011-9-26
pubmed:meshHeading
pubmed-meshheading:9101410-Amino Acid Sequence, pubmed-meshheading:9101410-Animals, pubmed-meshheading:9101410-Antigens, Neoplasm, pubmed-meshheading:9101410-Base Sequence, pubmed-meshheading:9101410-Cell Line, pubmed-meshheading:9101410-Cloning, Molecular, pubmed-meshheading:9101410-Genetic Code, pubmed-meshheading:9101410-Humans, pubmed-meshheading:9101410-Immunotherapy, pubmed-meshheading:9101410-MART-1 Antigen, pubmed-meshheading:9101410-Melanoma, pubmed-meshheading:9101410-Membrane Glycoproteins, pubmed-meshheading:9101410-Mice, pubmed-meshheading:9101410-Mice, Inbred C57BL, pubmed-meshheading:9101410-Molecular Sequence Data, pubmed-meshheading:9101410-Neoplasm Proteins, pubmed-meshheading:9101410-Sequence Homology, Amino Acid, pubmed-meshheading:9101410-Sequence Homology, Nucleic Acid, pubmed-meshheading:9101410-Tumor Cells, Cultured, pubmed-meshheading:9101410-gp100 Melanoma Antigen
pubmed:year
1997
pubmed:articleTitle
Cloning and characterization of the genes encoding the murine homologues of the human melanoma antigens MART1 and gp100.
pubmed:affiliation
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
pubmed:publicationType
Journal Article